ID   B6SEG5_9ACTN            Unreviewed;       306 AA.
AC   B6SEG5;
DT   16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT   16-DEC-2008, sequence version 1.
DT   27-MAR-2024, entry version 57.
DE   SubName: Full=AlnA {ECO:0000313|EMBL:ACI88875.1};
GN   Name=alnA {ECO:0000313|EMBL:ACI88875.1};
OS   Streptomyces sp. CM020.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=569580 {ECO:0000313|EMBL:ACI88875.1};
RN   [1] {ECO:0000313|EMBL:ACI88875.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CM020 {ECO:0000313|EMBL:ACI88875.1};
RX   PubMed=18940666; DOI=10.1016/j.chembiol.2008.07.022;
RA   Oja T., Palmu K., Lehmussola H., Lepparanta O., Hannikainen K., Niemi J.,
RA   Mantsala P., Metsa-Ketela M.;
RT   "Characterization of the alnumycin gene cluster reveals unusual gene
RT   products for pyran ring formation and dioxan biosynthesis.";
RL   Chem. Biol. 15:1046-1057(2008).
RN   [2] {ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9}
RP   X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 2-306 IN COMPLEX WITH CALCIUM.
RX   PubMed=23297194; DOI=10.1073/pnas.1207407110;
RA   Oja T., Niiranen L., Sandalova T., Klika K.D., Niemi J., Mantsala P.,
RA   Schneider G., Metsa-Ketela M.;
RT   "Structural basis for C-ribosylation in the alnumycin A biosynthetic
RT   pathway.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:1291-1296(2013).
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DR   EMBL; EU852062; ACI88875.1; -; Genomic_DNA.
DR   PDB; 4EX8; X-ray; 2.10 A; A=2-306.
DR   PDB; 4EX9; X-ray; 3.15 A; A=2-306.
DR   PDBsum; 4EX8; -.
DR   PDBsum; 4EX9; -.
DR   AlphaFoldDB; B6SEG5; -.
DR   SMR; B6SEG5; -.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004730; F:pseudouridylate synthase activity; IEA:InterPro.
DR   Gene3D; 3.40.1790.10; Indigoidine synthase domain; 1.
DR   InterPro; IPR022830; Indigdn_synthA-like.
DR   InterPro; IPR007342; PsuG.
DR   PANTHER; PTHR42909:SF1; PFKB DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR42909; ZGC:136858; 1.
DR   Pfam; PF04227; Indigoidine_A; 1.
DR   SUPFAM; SSF110581; Indigoidine synthase A-like; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9};
KW   Calcium {ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0007829|PDB:4EX8}.
FT   BINDING         29
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         106
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         130
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         135
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9"
FT   BINDING         138
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9"
FT   BINDING         140
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         142
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         159
FT                   /ligand="D-ribulose 5-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58121"
FT                   /evidence="ECO:0007829|PDB:4EX9"
FT   BINDING         169
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9"
FT   BINDING         172
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4EX8, ECO:0007829|PDB:4EX9"
SQ   SEQUENCE   306 AA;  31633 MW;  9509703572DAE228 CRC64;
     MERQPDQLLE VSDEIATALA ERRPVVALES SLITTDPSSE TASLIEKAVR GAGAVPATIG
     IAGGKLVVGL TDSLIERFAS TKGIPKISAR DIGGALAGGG LGATTVAGTI VIAERAGIQV
     FTTAGIGGVH RRGEDTLDIS PDLLQFRKTK MTVVSGGAKS ILDHRLTAEY LETAGVPVYG
     YRTDKLAAFV VREADVPVTR MDDLHTAARA AEAHWQVNGP GTVLLTSPID EQDAVDEAIV
     EAAIAEALAQ CDQEGIVGNA VSPYLMKALA RASGGMLPKA GRSLLLSTAR VAGEFSAALS
     AVQAER
//