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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) (Penicillium marneffei)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei131Substrate; via amide nitrogenBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Active sitei191Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei303NucleophileSequence analysis1
Binding sitei368SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:PMAA_057160
OrganismiTalaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) (Penicillium marneffei)
Taxonomic identifieri441960 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces
Proteomesi
  • UP000001294 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:PMAA_057160.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000039523122 – 1009Probable beta-galactosidase BAdd BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi266 ↔ 319By similarity
Glycosylationi375N-linked (GlcNAc...)Sequence analysis1
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1
Glycosylationi451N-linked (GlcNAc...)Sequence analysis1
Glycosylationi682N-linked (GlcNAc...)Sequence analysis1
Glycosylationi740N-linked (GlcNAc...)Sequence analysis1
Glycosylationi771N-linked (GlcNAc...)Sequence analysis1
Glycosylationi784N-linked (GlcNAc...)Sequence analysis1
Glycosylationi826N-linked (GlcNAc...)Sequence analysis1
Glycosylationi883N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi441960.XP_002150536.1.

Structurei

3D structure databases

ProteinModelPortaliB6QLF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B6QLF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLFTKIIV YFLLFASPLL ANQWPLHNNS LNDVVQWDHY SFEINGQRLF
60 70 80 90 100
VFAGEWHYWR IPVPELWIDI LEKIKAAGFT AFGIYVNWAY HAPNNYTVDF
110 120 130 140 150
ATGAHDITPI LKMAKDVGLY VLLRPGPYIN AEVNAGGFPL WVTTGEYGSL
160 170 180 190 200
RNDDSRYTAA WEPYFTKISE IASEYQITKG GNVVTYQIEN EFGDQWTGSP
210 220 230 240 250
SRRVQYEPAA KYMELLEANA RRNGIDIPLV ANEPNMRAIS WGKDWSNSSA
260 270 280 290 300
NVDVVGLDSY PSCWTCDLSV CTGTTGEYIA YQVIDYYGYF QETQPTMPSF
310 320 330 340 350
FAEFQGGSYN PWGGPVGGCP EDIGPDFANL FYRWNIGQRV TAINLYMLYG
360 370 380 390 400
GTNWGAIAAP VVASSYDYSS PISENRTIGA KYYETKLLTM FTRAARDLIV
410 420 430 440 450
TDLIGNGTQY STNPAIQTHV IRNPITNGTF YVTLHTISSS STDETFQLHV
460 470 480 490 500
NTSAGAFSIP RYGNSIRLNG HQSKIIVTDF QFGTHKLLYS TAEVLTYTVL
510 520 530 540 550
DGIPTLALWV PTGESGEFSV LGGKWASVLR CEWCSDIQFH PEQDQTSNPT
560 570 580 590 600
VSRLTVSFTQ GQGMSVIKLD TGLRVVLLDR ESAYYFWAPA LNSDPSVPED
610 620 630 640 650
QSVLVQGPHL VRSAKIVGST IRLTGDSAQT SPIEVFAPKQ VRIIFWNDKE
660 670 680 690 700
LKTSKTSYDS LQASLPQPAY VKLPLLGPWK YNGSLPEKAQ DYKDTSAAWI
710 720 730 740 750
SADHMKTSNP SPPATFPVLY ADEYGFHNSI RIWRGYFTGN ATGVVLKVQG
760 770 780 790 800
GYAFGFSGWL NGKLLGSYLG NASVEHSHLT LPFNVSHVST SSDNVLVIVH
810 820 830 840 850
DDTGHDETTG ALNPRGILEA TLLSDNSSSK FSHWRVAGTA GGETNIDPMR
860 870 880 890 900
GPYNEGGLYA ERMGWHLPGF NDNAWSDAGS KLNFTGADIK FYRTTVPLNI
910 920 930 940 950
PKGVDVSISF ELSACGTTNA FRSQLFVNGY QMGRFNPWVG NQIEFPVPPG
960 970 980 990 1000
ILDYTGDNTI GLSLWAQTED GACAIVDWKI NYVLGSSLDV TFNGEYLRPG

WTSERLQYS
Length:1,009
Mass (Da):111,835
Last modified:December 16, 2008 - v1
Checksum:iCCE39753CD678910
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS995903 Genomic DNA. Translation: EEA21927.1.
RefSeqiXP_002150536.1. XM_002150500.1.

Genome annotation databases

EnsemblFungiiEEA21927; EEA21927; PMAA_057160.
GeneIDi7028036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS995903 Genomic DNA. Translation: EEA21927.1.
RefSeqiXP_002150536.1. XM_002150500.1.

3D structure databases

ProteinModelPortaliB6QLF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441960.XP_002150536.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEEA21927; EEA21927; PMAA_057160.
GeneIDi7028036.

Organism-specific databases

EuPathDBiFungiDB:PMAA_057160.

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_TALMQ
AccessioniPrimary (citable) accession number: B6QLF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: December 16, 2008
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.