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Protein

Nitrilase

Gene

PMAA_032480

Organism
Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) (Penicillium marneffei)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that hydrolyzes preferentially 4-cyanopyridine.2 Publications

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.2 Publications

Kineticsi

  1. KM=7.9 mM for benzonitrile1 Publication
  2. KM=11.1 mM for 3-chlorobenzonitrile1 Publication
  3. KM=2.6 mM for 4-chlorobenzonitrile1 Publication
  4. KM=11.7 mM for 2-cyanopyridine1 Publication
  5. KM=34.4 mM for 3-cyanopyridine1 Publication
  6. KM=15.4 mM for 4-cyanopyridine1 Publication
  7. KM=9.1 mM for phenylacetonitrile1 Publication
  1. Vmax=0.16 µmol/min/mg enzyme toward benzonitrile1 Publication
  2. Vmax=1.9 µmol/min/mg enzyme toward 3-chlorobenzonitrile1 Publication
  3. Vmax=0.13 µmol/min/mg enzyme toward 4-chlorobenzonitrile1 Publication
  4. Vmax=0.46 µmol/min/mg enzyme toward 2-cyanopyridine1 Publication
  5. Vmax=0.21 µmol/min/mg enzyme toward 3-cyanopyridine1 Publication
  6. Vmax=7.3 µmol/min/mg enzyme toward 4-cyanopyridine1 Publication
  7. Vmax=0.1 µmol/min/mg enzyme toward phenylacetonitrile1 Publication

pH dependencei

Optimum pH is 6.5-8.5.1 Publication

Temperature dependencei

Optimum temperature is 30-35 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorPROSITE-ProRule annotation1
Active sitei127PROSITE-ProRule annotation1
Active sitei162NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.5.1. 9894.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase2 Publications (EC:3.5.5.12 Publications)
Gene namesi
ORF Names:PMAA_032480
OrganismiTalaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) (Penicillium marneffei)
Taxonomic identifieri441960 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces
Proteomesi
  • UP000001294 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:PMAA_032480.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004321781 – 322NitrilaseAdd BLAST322

Interactioni

Protein-protein interaction databases

STRINGi441960.XP_002144951.1.

Structurei

3D structure databases

ProteinModelPortaliB6Q5I3.
SMRiB6Q5I3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 283CN hydrolasePROSITE-ProRule annotationAdd BLAST279

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B6Q5I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIVRVGAV QSEPVWLDLE GSVDKTISLI EKAAADGVNV LGFPEVWIPG
60 70 80 90 100
YPWSMWTSAV INNSHIIHDY MNNSMRKDSP QMKRIQAAVK EAGMVVVLGY
110 120 130 140 150
SERDGASLYM AQSFIDPSGE IVHHRRKIKP THIERTIWGE GQAESLTCVI
160 170 180 190 200
DSPFGKVGGL NCWEHLQPLL RYYEYSQGVQ IHIASWPAEF EMPDPKKIAW
210 220 230 240 250
LYHETGEASY RASQFFAIEG QAFVLVASQI LTEANVERNN LTGNPVTKTP
260 270 280 290 300
GGGFSMIFGP DGKPLCEPVD AGAEAILTAD IDLRDIDKPK AFIDVVGHYA
310 320
RPDLLSLLVN PTVDKHVTTM KK
Length:322
Mass (Da):35,663
Last modified:December 16, 2008 - v1
Checksum:i14FF85AF1392E659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS995899 Genomic DNA. Translation: EEA28436.1.
RefSeqiXP_002144951.1. XM_002144915.1.

Genome annotation databases

EnsemblFungiiEEA28436; EEA28436; PMAA_032480.
GeneIDi7022697.

Similar proteinsi

Entry informationi

Entry nameiNIT_TALMQ
AccessioniPrimary (citable) accession number: B6Q5I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: December 16, 2008
Last modified: October 25, 2017
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families