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Protein

Lipoyl synthase, mitochondrial

Gene

SJAG_03553

Organism
Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (SJAG_01271)
  2. Lipoyl synthase, mitochondrial (SJAG_03553)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107Iron-sulfur 1 (4Fe-4S)1
Metal bindingi112Iron-sulfur 1 (4Fe-4S)1
Metal bindingi118Iron-sulfur 1 (4Fe-4S)1
Metal bindingi138Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi142Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi145Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:SJAG_03553
OrganismiSchizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
Taxonomic identifieri402676 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000001744 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:SJAG_03553.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 77MitochondrionAdd BLAST77
ChainiPRO_000039829178 – 377Lipoyl synthase, mitochondrialAdd BLAST300

Interactioni

Protein-protein interaction databases

STRINGi402676.XP_002174692.1.

Structurei

3D structure databases

ProteinModelPortaliB6K4J2.
SMRiB6K4J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672. Eukaryota.
COG0320. LUCA.
OrthoDBiEOG092C13O2.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B6K4J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRRGGRILN RRIFGAGISA FRVQGRKCAS DLSKALASGP KFSDFLKGDK
60 70 80 90 100
EEQLKPEEAY ELEENVVLPN GSVHKRLPSW LKTKVPLGTN FNKIKNDLRG
110 120 130 140 150
LNLHTVCEEA RCPNMGECWG GKDKTRATAT IMLMGDTCTR GCRFCSIKTS
160 170 180 190 200
RKPPPLDPNE PEKTAEAIKR WGLGYVVLTS VDRDDLADAG ASHIAKTISL
210 220 230 240 250
IKSKAPQVLV EALTPDFSGN LDLVSHVAKS GLDVFAHNVE TVEELTPFVR
260 270 280 290 300
DRRANFRQSL RVLKHAKSVS PHLITKTSIM LGLGETDEEI EATLGELRKN
310 320 330 340 350
EVDVVTFGQY MRPTKRHMKV QAYITPDKFE HWRKRAEELG FLYVASGPLV
360 370
RSSYKAGEFY LTNILRRRTA AKAQADH
Length:377
Mass (Da):42,163
Last modified:December 16, 2008 - v1
Checksum:i7424BAC10F1E8B2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KE651167 Genomic DNA. Translation: EEB08399.1.
RefSeqiXP_002174692.2. XM_002174656.2.

Genome annotation databases

GeneIDi7048812.

Similar proteinsi

Entry informationi

Entry nameiLIPA_SCHJY
AccessioniPrimary (citable) accession number: B6K4J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 16, 2008
Last modified: October 25, 2017
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families