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B6JNR5

- SYI_HELP2

UniProt

B6JNR5 - SYI_HELP2

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter pylori (strain P12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPYL570508:GJ8D-1437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HPP12_1395
OrganismiHelicobacter pylori (strain P12)
Taxonomic identifieri570508 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000008198: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_1000189170Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi570508.HPP12_1395.

Structurei

3D structure databases

ProteinModelPortaliB6JNR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTALWQIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6JNR5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQAFKR MQNRKDNHGD
60 70 80 90 100
FTLHDGPPYA NGHLHLGHAL NKILKDIVVK REYFKGNKIY YTPGWDCHGL
110 120 130 140 150
PIEQQILERL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD
160 170 180 190 200
FEDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE
210 220 230 240 250
VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL
260 270 280 290 300
KKDAVYALTQ KGYLVAKALH EKLAALGVVD SEIVHEFNSN DLEYLKATNP
310 320 330 340 350
LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLRY NLEVLMSVDE
360 370 380 390 400
RGCYDEGIIH NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH
410 420 430 440 450
CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALN AIEKVEFVPN
460 470 480 490 500
SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA
510 520 530 540 550
NLFEKKGCDV WWEYSVKDLL PPSYQEDAMH YEKVMHILDV WFDSGSTFKA
560 570 580 590 600
VLEDYHGEKG QSPSDVILEG SDQHRGWFQS SLLIGCILNN QAPFKKVITH
610 620 630 640 650
GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ
660 670 680 690 700
TFFIQTEQHY KKFRNTLKFL LANFSDMDLK NLERSHDFSP LDHFILEALE
710 720 730 740 750
TTSTGVNSAF EEHDFVKGLN ILMAFVTNEL SGIYLDACKD SLYCDSKNNE
760 770 780 790 800
KRQAIQMVLL AIASKLCYFL APILTHTIEE VLEHSQVLCA FLQAKDVFDL
810 820 830 840 850
KGISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE SVVKNSLECA
860 870 880 890 900
IEVKEKALRE NLIEELLMVS FVGVAKEKLS ETPAFTLFKA PFYKCPRCWR
910 920
FKSELENTPC KRCEEVLKER
Length:920
Mass (Da):106,139
Last modified:December 16, 2008 - v1
Checksum:iE004C252B8A57F3A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001217 Genomic DNA. Translation: ACJ08543.1.
RefSeqiYP_002302023.1. NC_011498.1.

Genome annotation databases

EnsemblBacteriaiACJ08543; ACJ08543; HPP12_1395.
GeneIDi7011150.
KEGGihpp:HPP12_1395.
PATRICi20610907. VBIHelPyl2824_1467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001217 Genomic DNA. Translation: ACJ08543.1 .
RefSeqi YP_002302023.1. NC_011498.1.

3D structure databases

ProteinModelPortali B6JNR5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 570508.HPP12_1395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ08543 ; ACJ08543 ; HPP12_1395 .
GeneIDi 7011150.
KEGGi hpp:HPP12_1395.
PATRICi 20610907. VBIHelPyl2824_1467.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi TALWQIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HPYL570508:GJ8D-1437-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Helicobacter pylori strain P12."
    Fischer W., Windhager L., Karnholz A., Zeiller M., Zimmer R., Haas R.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: P12.

Entry informationi

Entry nameiSYI_HELP2
AccessioniPrimary (citable) accession number: B6JNR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 16, 2008
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3