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B6JNR5 (SYI_HELP2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:HPP12_1395
OrganismHelicobacter pylori (strain P12) [Complete proteome] [HAMAP]
Taxonomic identifier570508 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 920920Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000189170

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8951Zinc By similarity
Metal binding8981Zinc By similarity
Metal binding9101Zinc By similarity
Metal binding9131Zinc By similarity
Binding site5691Aminoacyl-adenylate By similarity
Binding site6131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B6JNR5 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: E004C252B8A57F3A

FASTA920106,139
        10         20         30         40         50         60 
MKEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQAFKR MQNRKDNHGD FTLHDGPPYA 

        70         80         90        100        110        120 
NGHLHLGHAL NKILKDIVVK REYFKGNKIY YTPGWDCHGL PIEQQILERL EKEKTSLENP 

       130        140        150        160        170        180 
TLFREKCRDH AKKFLEIQKN EFLQLGVLGD FEDPYKTMDF KFEASIYRAL VEVAKKGLLK 

       190        200        210        220        230        240 
ERHKPIYWSY ACESALAEAE VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW 

       250        260        270        280        290        300 
TLYANVAIAL KKDAVYALTQ KGYLVAKALH EKLAALGVVD SEIVHEFNSN DLEYLKATNP 

       310        320        330        340        350        360 
LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLRY NLEVLMSVDE RGCYDEGIIH 

       370        380        390        400        410        420 
NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH CWRTHKPVIY RATTQWFILM 

       430        440        450        460        470        480 
DEPFIQNDGS QKTLREVALN AIEKVEFVPN SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF 

       490        500        510        520        530        540 
FIDKRTNKPC FESEVLEHVA NLFEKKGCDV WWEYSVKDLL PPSYQEDAMH YEKVMHILDV 

       550        560        570        580        590        600 
WFDSGSTFKA VLEDYHGEKG QSPSDVILEG SDQHRGWFQS SLLIGCILNN QAPFKKVITH 

       610        620        630        640        650        660 
GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ TFFIQTEQHY 

       670        680        690        700        710        720 
KKFRNTLKFL LANFSDMDLK NLERSHDFSP LDHFILEALE TTSTGVNSAF EEHDFVKGLN 

       730        740        750        760        770        780 
ILMAFVTNEL SGIYLDACKD SLYCDSKNNE KRQAIQMVLL AIASKLCYFL APILTHTIEE 

       790        800        810        820        830        840 
VLEHSQVLCA FLQAKDVFDL KGISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE 

       850        860        870        880        890        900 
SVVKNSLECA IEVKEKALRE NLIEELLMVS FVGVAKEKLS ETPAFTLFKA PFYKCPRCWR 

       910        920 
FKSELENTPC KRCEEVLKER 

« Hide

References

[1]"The complete genome sequence of Helicobacter pylori strain P12."
Fischer W., Windhager L., Karnholz A., Zeiller M., Zimmer R., Haas R.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: P12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001217 Genomic DNA. Translation: ACJ08543.1.
RefSeqYP_002302023.1. NC_011498.1.

3D structure databases

ProteinModelPortalB6JNR5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING570508.HPP12_1395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ08543; ACJ08543; HPP12_1395.
GeneID7011150.
KEGGhpp:HPP12_1395.
PATRIC20610907. VBIHelPyl2824_1467.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycHPYL570508:GJ8D-1437-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_HELP2
AccessionPrimary (citable) accession number: B6JNR5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries