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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Helicobacter pylori (strain P12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name:
DMAPP:tRNA dimethylallyltransferase
Short name:
DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
Short name:
IPTase
Gene namesi
Name:miaA
Ordered Locus Names:HPP12_1387
OrganismiHelicobacter pylori (strain P12)
Taxonomic identifieri570508 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000008198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003771871 – 274tRNA dimethylallyltransferaseAdd BLAST274

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei73Interaction with substrate tRNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliB6JNQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 12Interaction with substrate tRNABy similarity4

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Phylogenomic databases

HOGENOMiHOG000039995.
KOiK00791.
OMAiIFYATHT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

B6JNQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAEIFSLDS LSIYKDINIA SAKPSLKERK NIKHYALDHL NIDEKNNAQL
60 70 80 90 100
FKTLLEDAMR VSSKEILLIV GGSSFYLKSI LEGLSDTPKI SGEEVVKIER
110 120 130 140 150
EISALANPYA FLKSIDPTIA FKIHPNDTYR IHKALEIFYL THMPPSEYFK
160 170 180 190 200
ANPKKPFEHA ISLFALHIEK NALHNNIKQR TKNMLHSGLV EEIKALYAKY
210 220 230 240 250
PKDSQPFKAI GVKESVLFLE KQLTLKELEG AITFNPIKLA KRQNTFNKTQ
260 270
FNILYTGSVK EVRHAILKHS KSAY
Length:274
Mass (Da):31,242
Last modified:December 16, 2008 - v1
Checksum:i6360FFDBC6997980
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001217 Genomic DNA. Translation: ACJ08535.1.
RefSeqiWP_000338442.1. NC_011498.1.

Genome annotation databases

EnsemblBacteriaiACJ08535; ACJ08535; HPP12_1387.
KEGGihpp:HPP12_1387.
PATRICi20610891. VBIHelPyl2824_1459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001217 Genomic DNA. Translation: ACJ08535.1.
RefSeqiWP_000338442.1. NC_011498.1.

3D structure databases

ProteinModelPortaliB6JNQ7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ08535; ACJ08535; HPP12_1387.
KEGGihpp:HPP12_1387.
PATRICi20610891. VBIHelPyl2824_1459.

Phylogenomic databases

HOGENOMiHOG000039995.
KOiK00791.
OMAiIFYATHT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_HELP2
AccessioniPrimary (citable) accession number: B6JNQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.