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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Helicobacter pylori (strain P12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (HPP12_0859)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei260UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi191 – 194ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:HPP12_0858
OrganismiHelicobacter pylori (strain P12)
Taxonomic identifieri570508 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000008198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001481271 – 461Bifunctional protein HldEAdd BLAST461

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB6JM82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 311RibokinaseAdd BLAST311
Regioni332 – 461CytidylyltransferaseAdd BLAST130

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000237584.
KOiK03272.
OMAiILHKGHA.

Family and domain databases

CDDicd01172. RfaE_like. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE. 1 hit.
InterProiIPR023030. Bifunc_HldE.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.

Sequencei

Sequence statusi: Complete.

B6JM82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKILVVGDL IADYYLWGKS ERLSPEAPVP VLEVQKENKN LGGAANVANN
60 70 80 90 100
LISLKAKVFL CGVVGDDLEG KHFLNALKAR NIDTSGILTD KTRCTTLKTR
110 120 130 140 150
IIAQNQQIVR VDKEIKDPLN ADLRKRLLDF FTEKIQEIDG VILSDYNKGV
160 170 180 190 200
LDFELTQTMI ALANQHHKLI LCDPKGKDYS KYSHASLITP NRAELEHALH
210 220 230 240 250
LKLDSHANLS KALQILKETY QIAMPLVTLS EQGIAFLEKG ELVNCPTIAK
260 270 280 290 300
EVYDVTGAGD TVIASLTLSL LELMSLKDAC EFANAAAAVV VGKMGSALAS
310 320 330 340 350
LEEIALILNQ THPKILPLEK LLETLDQQKI VFTNGCFDLL HKGHASYLQK
360 370 380 390 400
AKALGDILIV GLNSDNSVKR LKGDKRPIVS EKDRAFLLAS LSCVDYVVVF
410 420 430 440 450
EEDTPIKLIQ ALKPDILVKG ADYLNKEVIG SEFAKETCLM EFEEGYSTSA
460
IIEKIKRTHN D
Length:461
Mass (Da):50,873
Last modified:December 16, 2008 - v1
Checksum:i5888F8196DF71E83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001217 Genomic DNA. Translation: ACJ08010.1.
RefSeqiWP_000723197.1. NC_011498.1.

Genome annotation databases

EnsemblBacteriaiACJ08010; ACJ08010; HPP12_0858.
KEGGihpp:HPP12_0858.
PATRICi20609721. VBIHelPyl2824_0899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001217 Genomic DNA. Translation: ACJ08010.1.
RefSeqiWP_000723197.1. NC_011498.1.

3D structure databases

ProteinModelPortaliB6JM82.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ08010; ACJ08010; HPP12_0858.
KEGGihpp:HPP12_0858.
PATRICi20609721. VBIHelPyl2824_0899.

Phylogenomic databases

HOGENOMiHOG000237584.
KOiK03272.
OMAiILHKGHA.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Family and domain databases

CDDicd01172. RfaE_like. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE. 1 hit.
InterProiIPR023030. Bifunc_HldE.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHLDE_HELP2
AccessioniPrimary (citable) accession number: B6JM82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.