B6JL62 (SYE1_HELP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	HPP12_0486

Organism

Helicobacter pylori (strain P12) [Complete proteome] [HAMAP]

Taxonomic identifier

570508 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367685
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6JL62 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: F15DBFF3C85C6F5D	FASTA	463	53,377
	10 20 30 40 50 60 MSLIVTRFAP SPTGYLHIGG LRTAIFNYLF ARANQGKFFL RIEDTDLSRN SIEAANAIIE 70 80 90 100 110 120 AFKWVGLEYD GEILYQSKRF EIYKEYIQKL LDEDKAYYCY MSKYELDALR EEQKARKETP 130 140 150 160 170 180 RYDNRYRDFK GTPPKGIEPV VRIKVPQNEV IGFNDGVKGE VRVNTNELDD FIIARSDGVP 190 200 210 220 230 240 TYNFVVTIDD ALMGITDVIR GDDHLSNTPK QIVLYKALNF KIPNFFHVPM ILNEEGQKLS 250 260 270 280 290 300 KRHGATNVMD YQERGYLKEA LVNFLARLGW SYQDKEIFSM QELLECFDPK DLNSSPSCFS 310 320 330 340 350 360 WHKLNWLNAH YLKNQSTQEL LKLLKPFSFS DLSHLNPAQL DRLLDALKER SQTLKELALK 370 380 390 400 410 420 IDEVLTAPIE YEEKVLKKLN QALVMPLLEK FKLELNTTNF NDESALENAM HQIIEEEKIK 430 440 450 460 AGSFMQPLRL ALLGKGGGIG LKEALFILGK TESVKRIEKF LKN

References

[1]	"The complete genome sequence of Helicobacter pylori strain P12." Fischer W., Windhager L., Karnholz A., Zeiller M., Zimmer R., Haas R. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: P12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001217 Genomic DNA. Translation: ACJ07640.1.
RefSeq	YP_002301120.1. NC_011498.1.
3D structure databases
ProteinModelPortal	B6JL62.
ModBase	Search...
Protein-protein interaction databases
STRING	B6JL62.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7010861.
GenomeReviews	Gene locus HPP12_0486 in contig CP001217_GR.
KEGG	hpp:HPP12_0486.
PATRIC	20608944. VBIHelPyl2824_0512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_HELP2

Accession

Primary (citable) accession number: B6JL62

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program