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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12MagnesiumUniRule annotation1
Metal bindingi72MagnesiumUniRule annotation1
Metal bindingi144MagnesiumUniRule annotation1
Metal bindingi147MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciOCAR504832:G1GTX-3146-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:OCAR_7302, OCA5_c08140
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
Proteomesi
  • UP000007730 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000905421 – 171Crossover junction endodeoxyribonuclease RuvCAdd BLAST171

Proteomic databases

PRIDEiB6JIZ7

Interactioni

Protein-protein interaction databases

STRINGi504832.OCA5_c08140

Structurei

3D structure databases

ProteinModelPortaliB6JIZ7
SMRiB6JIZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

B6JIZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISRTIRILG IDPGLRRTGW GVIDVEGNRL IFVACGTVES RESLPLSERL
60 70 80 90 100
LAIHEGLVRV VSEHRPLEAA VEQTFVNKDG AGTLKLGQAR GVAMLVPAMS
110 120 130 140 150
GISVAEYAPN LVKKTVVGAG HADKNQIQVM LKILLPKAAP PTPDAADALA
160 170
IAITHAHHRQ SAMLLRKVAA L
Length:171
Mass (Da):18,212
Last modified:December 16, 2008 - v1
Checksum:i2625B81AEEDE4A70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA Translation: ACI94406.1
CP002826 Genomic DNA Translation: AEI05536.1
RefSeqiWP_012564432.1, NC_015684.1

Genome annotation databases

EnsemblBacteriaiACI94406; ACI94406; OCAR_7302
AEI05536; AEI05536; OCA5_c08140
KEGGioca:OCAR_7302
ocg:OCA5_c08140
PATRICifig|504832.7.peg.860

Similar proteinsi

Entry informationi

Entry nameiRUVC_OLICO
AccessioniPrimary (citable) accession number: B6JIZ7
Secondary accession number(s): F8BZ45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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