B6JIZ7 (RUVC_OLICO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	OCAR_7302, OCA5_c08140

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha › Oligotropha carboxidovorans

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_1000090542
Sites
	Metal binding	12	1	Magnesium By similarity
	Metal binding	72	1	Magnesium By similarity
	Metal binding	144	1	Magnesium By similarity
	Metal binding	147	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6JIZ7 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 2625B81AEEDE4A70	FASTA	171	18,212
	10 20 30 40 50 60 MISRTIRILG IDPGLRRTGW GVIDVEGNRL IFVACGTVES RESLPLSERL LAIHEGLVRV 70 80 90 100 110 120 VSEHRPLEAA VEQTFVNKDG AGTLKLGQAR GVAMLVPAMS GISVAEYAPN LVKKTVVGAG 130 140 150 160 170 HADKNQIQVM LKILLPKAAP PTPDAADALA IAITHAHHRQ SAMLLRKVAA L

References

[1]	"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T." Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L. J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.
[2]	"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5." Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O. J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001196 Genomic DNA. Translation: ACI94406.1. CP002826 Genomic DNA. Translation: AEI05536.1.
RefSeq	YP_002290271.1. NC_011386.1. YP_004631777.1. NC_015684.1.
3D structure databases
ProteinModelPortal	B6JIZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	504832.OCAR_7302.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI94406; ACI94406; OCAR_7302. AEI05536; AEI05536; OCA5_c08140.
GeneID	10845946. 6992779.
KEGG	oca:OCAR_7302. ocg:OCA5_c08140.
PATRIC	22808397. VBIOliCar134280_3143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012181.
KO	K01159.
OMA	YTALQMK.
Enzyme and pathway databases
BioCyc	OCAR504832:GJPZ-814-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_OLICO

Accession

Primary (citable) accession number: B6JIZ7
Secondary accession number(s): F8BZ45

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program