B6JIX8 (LPXK_OLICO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	OCAR_7251, OCA5_c08640

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000123724
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B6JIX8 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 2F4AE4549F10D8D1	FASTA	337	35,601
	10 20 30 40 50 60 MREPRFWYGP VSLGSVLLAP IAACYGAVAA ARLAREGTRA GCPVICIGNY HVGGAGKTPT 70 80 90 100 110 120 TLRLVEILRE IGETPVVVSR GYGGTLAGPV RVNETHRAAD VGDEPKMMAR HVPVIVARDR 130 140 150 160 170 180 VEGAELARRE GASVVLLDDG FQNPALAKDA AVIVIDAARG LGNRMIFPAG PLRAPLAPQV 190 200 210 220 230 240 ARTNALIVIG EGHAADDIAQ GVVQRGGLVV RAAFVPEESS LARLRGARVL AFAGIGDPGR 250 260 270 280 290 300 FFATLAKHGV ELASRKEFAD HHPFTEAELK ALADEAQAGG LTLVTTEKDL ARIEGDPALA 310 320 330 AYAAQIVPFA VTLRVEDEGA LLDFLKARMR RARQARQ

References

[1]	"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T." Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L. J. Bacteriol. 190:5531-5532(2008) [PubMed: 18539730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.
[2]	"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5." Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O. J. Bacteriol. 193:5043-5043(2011) [PubMed: 21742883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001196 Genomic DNA. Translation: ACI94355.1. CP002826 Genomic DNA. Translation: AEI05586.1.
RefSeq	YP_002290220.1. NC_011386.1. YP_004631827.1. NC_015684.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B6JIX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10845996. 6992728.
GenomeReviews	Gene locus OCAR_7251 in contig CP001196_GR.
KEGG	oca:OCAR_7251.
PATRIC	22808293. VBIOliCar134280_3091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_OLICO

Accession

Primary (citable) accession number: B6JIX8
Secondary accession number(s): F8BZT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program