UniProtKB - B6JH82 (SYE_OLICO)
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Protein
Glutamate--tRNA ligase
Gene
gltX
Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Functioni
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation
Catalytic activityi
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 243 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-tRNA ligase activity Source: UniProtKB-EC
- tRNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- glutamyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | OCAR504832:G1GTX-1881-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)Alternative name(s): Glutamyl-tRNA synthetaseUniRule annotation Short name: GluRSUniRule annotation |
Gene namesi | Name:gltXUniRule annotation Ordered Locus Names:OCAR_5972, OCA5_c20520 |
Organismi | Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) |
Taxonomic identifieri | 504832 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000090094 | 1 – 473 | Glutamate--tRNA ligaseAdd BLAST | 473 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 504832.OCA5_c20520. |
Structurei
3D structure databases
ProteinModelPortali | B6JH82. |
SMRi | B6JH82. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 11 – 21 | "HIGH" regionUniRule annotationAdd BLAST | 11 | |
Motifi | 240 – 244 | "KMSKS" regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C20. Bacteria. COG0008. LUCA. |
HOGENOMi | HOG000252722. |
KOi | K01885. |
OMAi | AYYAFDT. |
OrthoDBi | POG091H021W. |
Family and domain databases
CDDi | cd00808. GluRS_core. 1 hit. |
Gene3Di | 1.10.10.350. 1 hit. 3.40.50.620. 1 hit. |
HAMAPi | MF_00022. Glu_tRNA_synth_type1. 1 hit. |
InterProi | View protein in InterPro IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_bac/mito. IPR000924. Glu/Gln-tRNA-synth. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR033910. GluRS_core. IPR014729. Rossmann-like_a/b/a_fold. |
Pfami | View protein in Pfam PF00749. tRNA-synt_1c. 1 hit. |
PRINTSi | PR00987. TRNASYNTHGLU. |
SUPFAMi | SSF48163. SSF48163. 1 hit. |
TIGRFAMsi | TIGR00464. gltX_bact. 1 hit. |
PROSITEi | View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit. |
i Sequence
Sequence statusi: Complete.
B6JH82-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEPVVTRFA PSPTGFLHIG GARTALFNWL YARKQGGKML LRIEDTDRER
60 70 80 90 100
STDAAIKAIL DGLNWLGIEW DGEVIYQFSR AARHREVAEQ LLAEGKAYRC
110 120 130 140 150
YATPEELTKM REAARAEGRA VRYDGRWRDR DPSEAPADVK PVIRLKAPQT
160 170 180 190 200
GETVIEDQVQ GRVVWQNENL DDLVLLRSDG TPTYMLAVVV DDHDMGVTHV
210 220 230 240 250
IRGDDHLINA ARQKHIYDAL GWTVPTMAHI PLIHGPDGSK LSKRHGALGV
260 270 280 290 300
EAYRTMGYLP AALRNYLVRL GWSHGDQEIF STSEMIEAFE LSGIGRSAAR
310 320 330 340 350
FDFAKLENLN GHYMRASGDA ELVKAFEDIL QFLPQGPALQ AKLNDTTRAQ
360 370 380 390 400
LLQAMPGLKE RAKTLLELID SAAYIFADRP LALDAKASAV LTPQVRALLG
410 420 430 440 450
ELRSSLANVT DWNAANTEAA MRAYAEKNNL KLGAVAQPLR AALTGRTTSP
460 470
GIFDVLAVLG RDDALARLQD QAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001196 Genomic DNA. Translation: ACI93092.1. CP002826 Genomic DNA. Translation: AEI06759.1. |
RefSeqi | WP_012563119.1. NC_015684.1. |
Genome annotation databases
EnsemblBacteriai | ACI93092; ACI93092; OCAR_5972. AEI06759; AEI06759; OCA5_c20520. |
KEGGi | oca:OCAR_5972. ocg:OCA5_c20520. |
PATRICi | fig|504832.7.peg.2172. |
Similar proteinsi
Entry informationi
Entry namei | SYE_OLICO | |
Accessioni | B6JH82Primary (citable) accession number: B6JH82 Secondary accession number(s): F8BW48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | December 16, 2008 | |
Last modified: | February 28, 2018 | |
This is version 57 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |