B6JGJ7 (SYD_OLICO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	OCAR_6334, OCA5_c17050

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000091020

Sequences

	Sequence		Length	Mass (Da)
	B6JGJ7 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 6D233F8AB6D364D7	FASTA	590	66,845
	10 20 30 40 50 60 MHRYRSHTCG ALRESDIDSN VRLSGWCHRI RDHGGLLFID LRDHYGITQC VVDPDSKAFR 70 80 90 100 110 120 LAETLRSEWV VKFDGRVRRR PDGTDNPELP TGAVEVFINE IEVLGEAAEL PMPVFGDQEY 130 140 150 160 170 180 PEEIRLKYRF LDLRRDRLHQ NIMTRGKIID SMRARMKEVG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDLE 250 260 270 280 290 300 MSFVTQEDVF AAMEPVITGV FEDFADGKPV TKNWPRIPFA EALRKYGTDK PDLRNPLIMQ 310 320 330 340 350 360 DVSEHFRGSG FKVFARMLEQ EGNEVWAIPG PAGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGGEGAGPLA NNIGPERTAA IRDQLGLKEG DAAFFVAGDP QKFWKFAGLA RTKLGEELNL 430 440 450 460 470 480 IDKDRFELAW IVDFPMYEYN EDEKKVDFSH NPFSMPQGGL EALQTQDPLT IKAFQYDIAC 490 500 510 520 530 540 NGYEIASGGI RNHKPAAMVK AFEIAGYGEE TVIERFGGMY RAFQYGAPPH GGMAAGVDRI 550 560 570 580 590 VMLLCGTNNL REISLFPMNQ RAEDLLMGAP SDVTTQQLRE LHIRLNLPQN

References

[1]	"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T." Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L. J. Bacteriol. 190:5531-5532(2008) [PubMed: 18539730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.
[2]	"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5." Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O. J. Bacteriol. 193:5043-5043(2011) [PubMed: 21742883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001196 Genomic DNA. Translation: ACI93447.1. CP002826 Genomic DNA. Translation: AEI06419.1.
RefSeq	YP_002289312.1. NC_011386.1. YP_004632660.1. NC_015684.1.
3D structure databases
ProteinModelPortal	B6JGJ7.
ModBase	Search...
Protein-protein interaction databases
STRING	B6JGJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10846837. 6990141.
GenomeReviews	Gene locus OCAR_6334 in contig CP001196_GR.
KEGG	oca:OCAR_6334.
PATRIC	22806509. VBIOliCar134280_2205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_OLICO

Accession

Primary (citable) accession number: B6JGJ7
Secondary accession number(s): F8BRN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program