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B6JFC1 (B6JFC1_OLICO) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase RuleBase RU003777 HAMAP-Rule MF_00168
EC=2.4.2.29 RuleBase RU003777 HAMAP-Rule MF_00168
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168 EMBL ACI93339.1
Ordered Locus Names:OCA5_c18030 EMBL AEI06517.1, OCAR_6226 EMBL ACI93339.1
OrganismOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP] EMBL ACI93339.1
Taxonomic identifier504832 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha ›

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. RuleBase RU003777 HAMAP-Rule MF_00168 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. RuleBase RU003777 HAMAP-Rule MF_00168 SAAS SAAS004803

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. RuleBase RU003777 HAMAP-Rule MF_00168

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site931Nucleophile By similarity HAMAP-Rule MF_00168
Binding site941Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
B6JFC1 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: B2914D8FBC9FCACC

FASTA37740,997
        10         20         30         40         50         60 
MSLPNSFRLL AADGTARTGV LETPHGVVRT PAFMPVGTAG AMKGIHWREI RESGADIVLG 

        70         80         90        100        110        120 
NTYHLMLRPT AERIAALGGL QKFTGWNGPM LTDSGGFQVM SLAKLRKVTE QGVRFKSHID 

       130        140        150        160        170        180 
GAVLDLSPER AIEVQRLFGS DIAMQLDECV RLPASRDEVD RAMQLSLRWA ERCKRAFEGA 

       190        200        210        220        230        240 
PQGHMLFGIV QGGDVAKLRR QSAAGLGEIG FHGYAIGGLA VGEPQDVMLA MIEEAAPLLP 

       250        260        270        280        290        300 
QDRPRYLMGV GTPDDMIEAV KRGIDMFDCV LPTRNGRHGH AFTRRGVINL RNARHADDPR 

       310        320        330        340        350        360 
PLDEESSWPP ARSYSRAYLH HLVKAGEALG AMLLSEINIA YFQSLMAGVR EAIAAGQFAD 

       370 
FRAATKAGWA AGDLPPR

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5 [Mississippi] and OM5 EMBL ACI93339.1.
[2]"Complete Genome Sequences of the Chemolithoautotrophic Oligotropha carboxidovorans Strains OM4 and OM5."
Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5 and OM5 EMBL AEI06517.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001196 Genomic DNA. Translation: ACI93339.1.
CP002826 Genomic DNA. Translation: AEI06517.1.
RefSeqYP_002289205.1. NC_011386.1.
YP_004632758.1. NC_015684.1.

3D structure databases

SMRB6JFC1. Positions 5-368.
ModBaseSearch...

Protein-protein interaction databases

STRING504832.OCAR_6226.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI93339; ACI93339; OCAR_6226.
AEI06517; AEI06517; OCA5_c18030.
GeneID10846935.
6989954.
KEGGoca:OCAR_6226.
ocg:OCA5_c18030.
PATRIC22806303. VBIOliCar134280_2102.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycOCAR504832:GJPZ-1803-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB6JFC1_OLICO
AccessionPrimary (citable) accession number: B6JFC1
Entry history
Integrated into UniProtKB/TrEMBL: December 16, 2008
Last sequence update: December 16, 2008
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info