B6JCQ3 (DUT_OLICO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	OCAR_4488, OCA5_c00460

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000094974
Regions
	Region	72 – 74	3	Substrate binding By similarity
	Region	89 – 91	3	Substrate binding By similarity
Sites
	Binding site	85	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6JCQ3 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 1343AF206F224CD5	FASTA	152	15,849
	10 20 30 40 50 60 MTEPLRIAVT QLPHGQGLPL PAYQTEHAAG LDLLAAVPED KPLAIAPGER ALVPTGLAIA 70 80 90 100 110 120 LPAGYEAQVR PRSGLAVKHG VTVLNAPGTI DADYRGEIGV PLINHGRETF IIRRGERIAQ 130 140 150 MVVAPVVQIA FEPVAELPMS ARGAGGFGST GR

References

[1]	"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T." Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L. J. Bacteriol. 190:5531-5532(2008) [PubMed: 18539730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.
[2]	"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5." Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O. J. Bacteriol. 193:5043-5043(2011) [PubMed: 21742883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001196 Genomic DNA. Translation: ACI91633.1. CP002826 Genomic DNA. Translation: AEI04780.1.
RefSeq	YP_002287498.1. NC_011386.1. YP_004631021.1. NC_015684.1.
3D structure databases
ProteinModelPortal	B6JCQ3.
ModBase	Search...
Protein-protein interaction databases
STRING	B6JCQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10845178. 6993241.
GenomeReviews	Gene locus OCAR_4488 in contig CP001196_GR.
KEGG	oca:OCAR_4488.
PATRIC	22802957. VBIOliCar134280_0464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG436079.
OMA	LDLRACI.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_OLICO

Accession

Primary (citable) accession number: B6JCQ3
Secondary accession number(s): F8C081

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program