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B6JB67 (TYSY_OLICO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:OCAR_5276, OCA5_c26960
OrganismOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]
Taxonomic identifier504832 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000089140

Sites

Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
B6JB67 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 7FDC464E6111F11A

FASTA26430,062
        10         20         30         40         50         60 
MHQYQDLLEH ILSDGAEKHD RTGTGTLSVF GHQMRFDLAA GFPMLTTKKL PFKAIVHELL 

        70         80         90        100        110        120 
WFLAGDTNVR YLQNNGVSIW DEWADANGDL GPVYGKQWRS WQAPDGRSID QITNLVQMIR 

       130        140        150        160        170        180 
TNPDSRRLIV TAWNPADVEQ MALPPCHCLF QFYVANGRLS CQLYQRSADV FLGVPFNIAS 

       190        200        210        220        230        240 
YALLMMMIAQ VTDLAPGHFV HTFGDAHLYS NHLDQARLQL TRAPRALPTL RINPAVRDIF 

       250        260 
GFRYEDFKLE NYDPHPHIKA EVAV 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.
[2]"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001196 Genomic DNA. Translation: ACI92408.1.
CP002826 Genomic DNA. Translation: AEI07390.1.
RefSeqYP_002288273.1. NC_011386.1.
YP_004633631.1. NC_015684.1.

3D structure databases

ProteinModelPortalB6JB67.
SMRB6JB67. Positions 1-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING504832.OCAR_5276.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI92408; ACI92408; OCAR_5276.
AEI07390; AEI07390; OCA5_c26960.
GeneID10847828.
6991263.
KEGGoca:OCAR_5276.
ocg:OCA5_c26960.
PATRIC22804477. VBIOliCar134280_1209.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycOCAR504832:GJPZ-2696-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_OLICO
AccessionPrimary (citable) accession number: B6JB67
Secondary accession number(s): F8BV35
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways