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B6JAT0 (ASSY_OLICO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:OCAR_4294, OCA5_c02270
OrganismOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]
Taxonomic identifier504832 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00581

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00581

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00581

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00581.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Argininosuccinate synthase HAMAP-Rule MF_00581
PRO_1000129758

Regions

Nucleotide binding17 – 259ATP By similarity

Sites

Binding site431ATP By similarity
Binding site991Citrulline By similarity
Binding site1291ATP; via amide nitrogen By similarity
Binding site1311Aspartate By similarity
Binding site1311ATP By similarity
Binding site1351Aspartate By similarity
Binding site1351Citrulline By similarity
Binding site1361Aspartate By similarity
Binding site1361ATP By similarity
Binding site1391Citrulline By similarity
Binding site1921Citrulline By similarity
Binding site1941ATP By similarity
Binding site2011Citrulline By similarity
Binding site2031Citrulline By similarity
Binding site2801Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
B6JAT0 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 30FE17AD28AC5D17

FASTA44549,388
        10         20         30         40         50         60 
MSTILKSLPK GENVGIAFSG GLDTSAALLW MKQKGARVFA YTANLGQPDE ADYDEIPRKA 

        70         80         90        100        110        120 
LEFGAEKARL VDCRTQLVHE GIAAIQSGAF HVSTGGIAYF NTTPLGRAVT GTMLVSAMKE 

       130        140        150        160        170        180 
DGVNIWGDGS TYKGNDIERF YRYGLLTNPE LRIYKPWLDQ QFIDELGGRA EMSAFMTAHG 

       190        200        210        220        230        240 
FAYKMSAEKA YSTDSNILGA THEAKDLEHL DSGIKIVNPI MGVPFWRDDC AVKAEKVSVR 

       250        260        270        280        290        300 
FEEGQPVALN GQTFTDPVAL FLEANTIGGR HGLGMSDQIE NRIIEAKSRG IYEAPAMALL 

       310        320        330        340        350        360 
HIAYERLVTG IHNEDTIEQY RISGMRLGRL LYQGRWFDSQ ALMLRETAQR WVARAITGEV 

       370        380        390        400        410        420 
TLELRRGNDY SILNTESPNL TYAPERLSME KVEDAAFTPG DRIGQLTMRN LDISDTRRKL 

       430        440 
NLYTQTGLLS AEQGSHIPRL DNDKS 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.
[2]"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001196 Genomic DNA. Translation: ACI91443.1.
CP002826 Genomic DNA. Translation: AEI04956.1.
RefSeqYP_002287308.1. NC_011386.1.
YP_004631197.1. NC_015684.1.

3D structure databases

ProteinModelPortalB6JAT0.
SMRB6JAT0. Positions 2-442.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING504832.OCAR_4294.

Proteomic databases

PRIDEB6JAT0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI91443; ACI91443; OCAR_4294.
AEI04956; AEI04956; OCA5_c02270.
GeneID10845359.
6993051.
KEGGoca:OCAR_4294.
ocg:OCA5_c02270.
PATRIC22802573. VBIOliCar134280_0277.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230094.
KOK01940.
OMARIVEPIM.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycOCAR504832:GJPZ-227-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00581. Arg_succ_synth_type2.
InterProIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_OLICO
AccessionPrimary (citable) accession number: B6JAT0
Secondary accession number(s): F8BSS9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 16, 2008
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways