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Protein

Argininosuccinate synthase

Gene

argG

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATPUniRule annotation
Binding sitei99 – 991CitrullineUniRule annotation
Binding sitei129 – 1291ATP; via amide nitrogenUniRule annotation
Binding sitei131 – 1311AspartateUniRule annotation
Binding sitei131 – 1311ATPUniRule annotation
Binding sitei135 – 1351AspartateUniRule annotation
Binding sitei135 – 1351CitrullineUniRule annotation
Binding sitei136 – 1361AspartateUniRule annotation
Binding sitei136 – 1361ATPUniRule annotation
Binding sitei139 – 1391CitrullineUniRule annotation
Binding sitei192 – 1921CitrullineUniRule annotation
Binding sitei194 – 1941ATPUniRule annotation
Binding sitei201 – 2011CitrullineUniRule annotation
Binding sitei203 – 2031CitrullineUniRule annotation
Binding sitei280 – 2801CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOCAR504832:GJPZ-227-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:OCAR_4294, OCA5_c02270
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
ProteomesiUP000007730 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Argininosuccinate synthasePRO_1000129758Add
BLAST

Proteomic databases

PRIDEiB6JAT0.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi504832.OCA5_c02270.

Structurei

3D structure databases

ProteinModelPortaliB6JAT0.
SMRiB6JAT0. Positions 2-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6JAT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTILKSLPK GENVGIAFSG GLDTSAALLW MKQKGARVFA YTANLGQPDE
60 70 80 90 100
ADYDEIPRKA LEFGAEKARL VDCRTQLVHE GIAAIQSGAF HVSTGGIAYF
110 120 130 140 150
NTTPLGRAVT GTMLVSAMKE DGVNIWGDGS TYKGNDIERF YRYGLLTNPE
160 170 180 190 200
LRIYKPWLDQ QFIDELGGRA EMSAFMTAHG FAYKMSAEKA YSTDSNILGA
210 220 230 240 250
THEAKDLEHL DSGIKIVNPI MGVPFWRDDC AVKAEKVSVR FEEGQPVALN
260 270 280 290 300
GQTFTDPVAL FLEANTIGGR HGLGMSDQIE NRIIEAKSRG IYEAPAMALL
310 320 330 340 350
HIAYERLVTG IHNEDTIEQY RISGMRLGRL LYQGRWFDSQ ALMLRETAQR
360 370 380 390 400
WVARAITGEV TLELRRGNDY SILNTESPNL TYAPERLSME KVEDAAFTPG
410 420 430 440
DRIGQLTMRN LDISDTRRKL NLYTQTGLLS AEQGSHIPRL DNDKS
Length:445
Mass (Da):49,388
Last modified:December 16, 2008 - v1
Checksum:i30FE17AD28AC5D17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91443.1.
CP002826 Genomic DNA. Translation: AEI04956.1.
RefSeqiWP_012561474.1. NC_015684.1.

Genome annotation databases

EnsemblBacteriaiACI91443; ACI91443; OCAR_4294.
AEI04956; AEI04956; OCA5_c02270.
KEGGioca:OCAR_4294.
ocg:OCA5_c02270.
PATRICi22802573. VBIOliCar134280_0277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91443.1.
CP002826 Genomic DNA. Translation: AEI04956.1.
RefSeqiWP_012561474.1. NC_015684.1.

3D structure databases

ProteinModelPortaliB6JAT0.
SMRiB6JAT0. Positions 2-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi504832.OCA5_c02270.

Proteomic databases

PRIDEiB6JAT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI91443; ACI91443; OCAR_4294.
AEI04956; AEI04956; OCA5_c02270.
KEGGioca:OCAR_4294.
ocg:OCA5_c02270.
PATRICi22802573. VBIOliCar134280_0277.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciOCAR504832:GJPZ-227-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
    Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
    J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49405 / DSM 1227 / OM5.
  2. "Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
    Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
    J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49405 / DSM 1227 / OM5.

Entry informationi

Entry nameiASSY_OLICO
AccessioniPrimary (citable) accession number: B6JAT0
Secondary accession number(s): F8BSS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 16, 2008
Last modified: July 22, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.