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B6JAI4 (SYL_OLICO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:OCAR_4363, OCA5_c01660
OrganismOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP]
Taxonomic identifier504832 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 879879Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091340

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B6JAI4 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 9C29A2C1EF90A0A5

FASTA87998,025
        10         20         30         40         50         60 
MSTERYNARE VEPRWQQIWD EKGVFASRND DSRPKYYVLE MFPYPSGRIH MGHVRNYTMG 

        70         80         90        100        110        120 
DVVARYRRAK GHNVLHPMGW DAFGMPAENA AMQNKTHPAK WTYANIAAMK KQLKSMGLSL 

       130        140        150        160        170        180 
DWAREIATCD PSYYKHQQRM FLDFLKAGLV ERKQSKVNWD PVDQTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKISDYS EELLTALDTL DRWPEKVRLM QKNWIGRSEG LLVRFALDAA 

       250        260        270        280        290        300 
TAPAGESEVE VFTTRPDTLF GAKFVALSPD HPLAAEVAKA NPKLEAFIAE CHRHGTAQAE 

       310        320        330        340        350        360 
IDTAEKLGFD TGLRARHPFD PDWLLPVYVA NFVLMDYGTG AIFGCPAHDQ RDLDFVNKYG 

       370        380        390        400        410        420 
LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT IADAKEDVAK RLETATLPRA 

       430        440        450        460        470        480 
NGGGNAPVAK RQVNYRLRDW GISRQRYWGC PIPIIHCESC GIVPVPVKDL PVKLPDDIEF 

       490        500        510        520        530        540 
DRPGNPLDRH PTWKHVACPQ CGGKARRETD TMDTFVDSSW YFARFTDPWN ENAPTTRKVV 

       550        560        570        580        590        600 
DAMMPVDQYI GGIEHAILHL LYSRFFTRAM KATGHVGFDE PFAGLFTQGM VVHETYKNAD 

       610        620        630        640        650        660 
GSWAAPSEIK IEGTGDARHA TLIDTGAPVE IGSIEKMSKS KRNTIDPDDI IGTYGADTAR 

       670        680        690        700        710        720 
WFMLSDSPPD RDVIWSEEGV QGANRFVQRV WRLVNLAAPH LPKDSAAAGT SADTKPLRST 

       730        740        750        760        770        780 
AHRTLADVSQ AIERLRFNTA IAKLYAFVGP LNEAIDDPRL KADPAWAASV REALDMLVRM 

       790        800        810        820        830        840 
IAPMMPHLAE QCWEALGGQG LVSEAAWPEV DPVLLVEDTI TLPVQINGKK RADVTVGRNA 

       850        860        870 
PNPEIEAAVL ALDAVKTALA GATPRKIIVV PQRIVNVVV 

« Hide

References

[1]"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.
[2]"Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49405 / DSM 1227 / OM5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001196 Genomic DNA. Translation: ACI91509.1.
CP002826 Genomic DNA. Translation: AEI04898.1.
RefSeqYP_002287374.1. NC_011386.1.
YP_004631139.1. NC_015684.1.

3D structure databases

ProteinModelPortalB6JAI4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING504832.OCAR_4363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI91509; ACI91509; OCAR_4363.
AEI04898; AEI04898; OCA5_c01660.
GeneID10845298.
6993117.
KEGGoca:OCAR_4363.
ocg:OCA5_c01660.
PATRIC22802705. VBIOliCar134280_0340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycOCAR504832:GJPZ-166-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_OLICO
AccessionPrimary (citable) accession number: B6JAI4
Secondary accession number(s): F8BS31
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries