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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOCAR504832:GJPZ-166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:OCAR_4363, OCA5_c01660
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
ProteomesiUP000007730 Componenti: Chromosome UP000001678 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 879879Leucine--tRNA ligasePRO_1000091340Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi504832.OCAR_4363.

Structurei

3D structure databases

ProteinModelPortaliB6JAI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6JAI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTERYNARE VEPRWQQIWD EKGVFASRND DSRPKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVVARYRRAK GHNVLHPMGW DAFGMPAENA AMQNKTHPAK
110 120 130 140 150
WTYANIAAMK KQLKSMGLSL DWAREIATCD PSYYKHQQRM FLDFLKAGLV
160 170 180 190 200
ERKQSKVNWD PVDQTVLANE QVIDGRGWRS GALVEQRELT QWFFKISDYS
210 220 230 240 250
EELLTALDTL DRWPEKVRLM QKNWIGRSEG LLVRFALDAA TAPAGESEVE
260 270 280 290 300
VFTTRPDTLF GAKFVALSPD HPLAAEVAKA NPKLEAFIAE CHRHGTAQAE
310 320 330 340 350
IDTAEKLGFD TGLRARHPFD PDWLLPVYVA NFVLMDYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYG LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT
410 420 430 440 450
IADAKEDVAK RLETATLPRA NGGGNAPVAK RQVNYRLRDW GISRQRYWGC
460 470 480 490 500
PIPIIHCESC GIVPVPVKDL PVKLPDDIEF DRPGNPLDRH PTWKHVACPQ
510 520 530 540 550
CGGKARRETD TMDTFVDSSW YFARFTDPWN ENAPTTRKVV DAMMPVDQYI
560 570 580 590 600
GGIEHAILHL LYSRFFTRAM KATGHVGFDE PFAGLFTQGM VVHETYKNAD
610 620 630 640 650
GSWAAPSEIK IEGTGDARHA TLIDTGAPVE IGSIEKMSKS KRNTIDPDDI
660 670 680 690 700
IGTYGADTAR WFMLSDSPPD RDVIWSEEGV QGANRFVQRV WRLVNLAAPH
710 720 730 740 750
LPKDSAAAGT SADTKPLRST AHRTLADVSQ AIERLRFNTA IAKLYAFVGP
760 770 780 790 800
LNEAIDDPRL KADPAWAASV REALDMLVRM IAPMMPHLAE QCWEALGGQG
810 820 830 840 850
LVSEAAWPEV DPVLLVEDTI TLPVQINGKK RADVTVGRNA PNPEIEAAVL
860 870
ALDAVKTALA GATPRKIIVV PQRIVNVVV
Length:879
Mass (Da):98,025
Last modified:December 16, 2008 - v1
Checksum:i9C29A2C1EF90A0A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91509.1.
CP002826 Genomic DNA. Translation: AEI04898.1.
RefSeqiYP_002287374.1. NC_011386.1.
YP_004631139.1. NC_015684.1.

Genome annotation databases

EnsemblBacteriaiACI91509; ACI91509; OCAR_4363.
AEI04898; AEI04898; OCA5_c01660.
KEGGioca:OCAR_4363.
ocg:OCA5_c01660.
PATRICi22802705. VBIOliCar134280_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91509.1.
CP002826 Genomic DNA. Translation: AEI04898.1.
RefSeqiYP_002287374.1. NC_011386.1.
YP_004631139.1. NC_015684.1.

3D structure databases

ProteinModelPortaliB6JAI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi504832.OCAR_4363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI91509; ACI91509; OCAR_4363.
AEI04898; AEI04898; OCA5_c01660.
KEGGioca:OCAR_4363.
ocg:OCA5_c01660.
PATRICi22802705. VBIOliCar134280_0340.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciOCAR504832:GJPZ-166-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T."
    Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.
    J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49405 / DSM 1227 / OM5.
  2. "Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5."
    Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O.
    J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49405 / DSM 1227 / OM5.

Entry informationi

Entry nameiSYL_OLICO
AccessioniPrimary (citable) accession number: B6JAI4
Secondary accession number(s): F8BS31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: April 1, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.