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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei639ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:OCAR_4363, OCA5_c01660
OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Taxonomic identifieri504832 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
Proteomesi
  • UP000007730 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000913401 – 879Leucine--tRNA ligaseAdd BLAST879

Proteomic databases

PRIDEiB6JAI4.

Interactioni

Protein-protein interaction databases

STRINGi504832.OCA5_c01660.

Structurei

3D structure databases

ProteinModelPortaliB6JAI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 53"HIGH" regionAdd BLAST11
Motifi636 – 640"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6JAI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTERYNARE VEPRWQQIWD EKGVFASRND DSRPKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVVARYRRAK GHNVLHPMGW DAFGMPAENA AMQNKTHPAK
110 120 130 140 150
WTYANIAAMK KQLKSMGLSL DWAREIATCD PSYYKHQQRM FLDFLKAGLV
160 170 180 190 200
ERKQSKVNWD PVDQTVLANE QVIDGRGWRS GALVEQRELT QWFFKISDYS
210 220 230 240 250
EELLTALDTL DRWPEKVRLM QKNWIGRSEG LLVRFALDAA TAPAGESEVE
260 270 280 290 300
VFTTRPDTLF GAKFVALSPD HPLAAEVAKA NPKLEAFIAE CHRHGTAQAE
310 320 330 340 350
IDTAEKLGFD TGLRARHPFD PDWLLPVYVA NFVLMDYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYG LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT
410 420 430 440 450
IADAKEDVAK RLETATLPRA NGGGNAPVAK RQVNYRLRDW GISRQRYWGC
460 470 480 490 500
PIPIIHCESC GIVPVPVKDL PVKLPDDIEF DRPGNPLDRH PTWKHVACPQ
510 520 530 540 550
CGGKARRETD TMDTFVDSSW YFARFTDPWN ENAPTTRKVV DAMMPVDQYI
560 570 580 590 600
GGIEHAILHL LYSRFFTRAM KATGHVGFDE PFAGLFTQGM VVHETYKNAD
610 620 630 640 650
GSWAAPSEIK IEGTGDARHA TLIDTGAPVE IGSIEKMSKS KRNTIDPDDI
660 670 680 690 700
IGTYGADTAR WFMLSDSPPD RDVIWSEEGV QGANRFVQRV WRLVNLAAPH
710 720 730 740 750
LPKDSAAAGT SADTKPLRST AHRTLADVSQ AIERLRFNTA IAKLYAFVGP
760 770 780 790 800
LNEAIDDPRL KADPAWAASV REALDMLVRM IAPMMPHLAE QCWEALGGQG
810 820 830 840 850
LVSEAAWPEV DPVLLVEDTI TLPVQINGKK RADVTVGRNA PNPEIEAAVL
860 870
ALDAVKTALA GATPRKIIVV PQRIVNVVV
Length:879
Mass (Da):98,025
Last modified:December 16, 2008 - v1
Checksum:i9C29A2C1EF90A0A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91509.1.
CP002826 Genomic DNA. Translation: AEI04898.1.
RefSeqiWP_012561540.1. NC_015684.1.

Genome annotation databases

EnsemblBacteriaiACI91509; ACI91509; OCAR_4363.
AEI04898; AEI04898; OCA5_c01660.
KEGGioca:OCAR_4363.
ocg:OCA5_c01660.
PATRICi22802705. VBIOliCar134280_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001196 Genomic DNA. Translation: ACI91509.1.
CP002826 Genomic DNA. Translation: AEI04898.1.
RefSeqiWP_012561540.1. NC_015684.1.

3D structure databases

ProteinModelPortaliB6JAI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi504832.OCA5_c01660.

Proteomic databases

PRIDEiB6JAI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI91509; ACI91509; OCAR_4363.
AEI04898; AEI04898; OCA5_c01660.
KEGGioca:OCAR_4363.
ocg:OCA5_c01660.
PATRICi22802705. VBIOliCar134280_0340.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_OLICO
AccessioniPrimary (citable) accession number: B6JAI4
Secondary accession number(s): F8BS31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.