B6JA10 (B6JA10_OLICO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374
EC=3.1.2.6 HAMAP-Rule MF_01374

Alternative name(s):
Glyoxalase II HAMAP-Rule MF_01374

Gene names

Name:	gloB HAMAP-Rule MF_01374 EMBL ACI91253.1
Ordered Locus Names:	OCA5_c04080 EMBL AEI05133.1, OCAR_4102 EMBL ACI91253.1

Organism

Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) [Complete proteome] [HAMAP] EMBL ACI91253.1

Taxonomic identifier

504832 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Oligotropha › Oligotropha carboxidovorans

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. SAAS SAAS017782 HAMAP-Rule MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. SAAS SAAS017782 HAMAP-Rule MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. SAAS SAAS017782 HAMAP-Rule MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374
Subunit structure	Monomer By similarity. SAAS SAAS017782 HAMAP-Rule MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP-Rule MF_01374

Ontologies

Keywords
Ligand	Metal-binding SAAS SAAS017782 HAMAP-Rule MF_01374 Zinc SAAS SAAS017782 HAMAP-Rule MF_01374
Molecular function	Hydrolase SAAS SAAS017782 HAMAP-Rule MF_01374 EMBL ACI91253.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	56	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	58	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	60	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	114	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	133	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	133	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	171	1	Zinc 2 By similarity HAMAP-Rule MF_01374

Sequences

	Sequence		Length	Mass (Da)
	B6JA10 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: DF6AEA136C74336C	FASTA	255	28,141
	10 20 30 40 50 60 MAAEIRVFPC LSDNIGYLIH DPVTKATAAI DAPDAKPIIA ALEREGWRLT DLLITHHHAD 70 80 90 100 110 120 HTDGIAELKA KYKCRVVGPY EKGVRIDGLD VHVKEGDTVK VGNLVARVFE TPGHTLDHIS 130 140 150 160 170 180 YMFDDAHALF CADVIFSIGC GRVIEGTYPM MWESLLKLRA LPNDTRVYCG HEYTASNVKF 190 200 210 220 230 240 ALGIEPNNPA LKKRAEEVTK LRAEGKLTIP SLMRDEKEAN VFLRADVPAV AAALGMNGKT 250 PAEVFGEIRE RKNRA

References

[1]	"Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T." Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L. J. Bacteriol. 190:5531-5532(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5 [Mississippi] and OM5 EMBL ACI91253.1.
[2]	"Complete Genome Sequences of the Chemolithoautotrophic Oligotropha carboxidovorans Strains OM4 and OM5." Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G., Meyer O. J. Bacteriol. 193:5043-5043(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49405 / DSM 1227 / OM5 and OM5 EMBL AEI05133.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001196 Genomic DNA. Translation: ACI91253.1. CP002826 Genomic DNA. Translation: AEI05133.1.
RefSeq	YP_002287118.1. NC_011386.1. YP_004631374.1. NC_015684.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	504832.OCAR_4102.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI91253; ACI91253; OCAR_4102. AEI05133; AEI05133; OCA5_c04080.
GeneID	10845540. 6991565.
KEGG	oca:OCAR_4102. ocg:OCA5_c04080.
PATRIC	22802211. VBIOliCar134280_0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HOG000058041.
KO	K01069.
OMA	PFMRVHE.
ProtClustDB	CLSK818037.
Enzyme and pathway databases
BioCyc	OCAR504832:GJPZ-408-MONOMER.
UniPathway	UPA00619; UER00676.
Family and domain databases
Gene3D	3.60.15.10. 1 hit.
HAMAP	MF_01374. Glyoxalase_2.
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PIRSF	PIRSF005457. Glx. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B6JA10_OLICO

Accession

Primary (citable) accession number: B6JA10

Entry history

Integrated into UniProtKB/TrEMBL:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)