B6J7I2 (TRPA_COXB1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	CbuK_1023

Organism

Coxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154)) [Complete proteome] [HAMAP]

Taxonomic identifier

434924 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Tryptophan synthase alpha chain HAMAP-Rule MF_00131		PRO_1000095707
Sites
	Active site	44	1	Proton acceptor By similarity
	Active site	55	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6J7I2 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 0B015A8562301435	FASTA	256	27,519
	10 20 30 40 50 60 MNRIEQQFKK SPAYVAYLTA GDGGLERSLE SLLALAKGGV NILEVGVPFS DPVADGPVIQ 70 80 90 100 110 120 EASIRALAQG TTLHDVLTLI TSFRQHSEIP IILFTYFNPL LAAGDKIYQQ MKSAGVDGCL 130 140 150 160 170 180 VVDLPVEEAA PHLTACKTAK IAPILLISPS TTQERLKKIN EHGEGMLYYV CRPGTTGVRA 190 200 210 220 230 240 TLPENFPAKM NQIKSMTSLP IVTGFGIANR KMAAQALQYA DGFVIGSLFV KAIAEGISKN 250 ALTRLAQSLN PHYPNP

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CbuK_Q154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001020 Genomic DNA. Translation: ACJ20231.1.
RefSeq	YP_002305376.1. NC_011528.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	434924.CbuK_1023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ20231; ACJ20231; CbuK_1023.
GeneID	7015536.
KEGG	cbc:CbuK_1023.
PATRIC	17917503. VBICoxBur77120_1050.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0159.
HOGENOM	HOG000223815.
KO	K01695.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	CBUR434924:GHWU-1021-MONOMER.
UniPathway	UPA00035; UER00044.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00131. Trp_synth_alpha.
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. trpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_COXB1

Accession

Primary (citable) accession number: B6J7I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program