B6J6H8 (METE_COXB1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CbuK_2095

Organism

Coxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154)) [Complete proteome] [HAMAP]

Taxonomic identifier

434924 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 775	775	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097822
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6J6H8 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 5FBC2B4A170D13DE	FASTA	775	88,391
	10 20 30 40 50 60 MVYAHNLGFP RIGIKREMKK TVEAYWRGEI SQQQLQQQAI ELQLTNWKIQ AEAGVDLIPV 70 80 90 100 110 120 GDFSWYDHVL DMAVRVGAIP SRFKALNSNI TDTMFCMARG QAPNGIETSA CEMTKWFDTN 130 140 150 160 170 180 YHYIVPEFTT NQSFELHHDD LFKSTKLALE NNYRAKPVIL GPLSFLWLGK CKGESFNKLL 190 200 210 220 230 240 LLEKLLPVYA EIFEQLSSLG VEWVQVDEPI LVLDLPPEWQ QAFLTTYQQL NFFNLKCLLA 250 260 270 280 290 300 TYFGSLDDNL SLTCQLPVDG LHIDYCRAPD QLDSVLSQLP AEKILSVGII DGRNIWCNDL 310 320 330 340 350 360 NRSLTLLENI QSSLGDRLWV APSCSLLHVP IDLDQENKLD VELKSWFAFA KQKVAEAAFL 370 380 390 400 410 420 TRGLREGRES IGAELKKNEE VIISRKTSKR IHNPNVEKKA ASVTERLMRR QHEHSIRKNK 430 440 450 460 470 480 QTAQLNLPLF PTTTIGSFPQ TSQIRCLRRD YKQGKIDDAL YEEKIRQEIA EVIGIQVKLG 490 500 510 520 530 540 LDVLVHGEPE RNDMVEYFGE LLDGIAITSN GWVQSYGSRC VKPPIIFGDV SRERPMTLRW 550 560 570 580 590 600 IEYAQSLTTK SVKGMLTGPV TILAWSFVRD DQPRSQTAKQ IALALRDEVQ DLERSGVRVI 610 620 630 640 650 660 QIDEPAFREC LPLRKAAWQD YLEWAVKCFR LASCGVKDET QIHTHMCYSE FNDIIEAIAA 670 680 690 700 710 720 LDADVITIES SRSETEILKS FEKFAYPNDI GPGIYDIHSP RIPRVAEIEE LAVRALQYIP 730 740 750 760 770 IERLWINPDC GLKTRNWEET KEALSRMVDA AKHLRKAFSS EKTPTIDLEL QPAST

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CbuK_Q154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001020 Genomic DNA. Translation: ACJ21188.1.
RefSeq	YP_002306333.1. NC_011528.1.
3D structure databases
ProteinModelPortal	B6J6H8.
ModBase	Search...
Protein-protein interaction databases
STRING	B6J6H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7016608.
GenomeReviews	Gene locus CbuK_2095 in contig CP001020_GR.
KEGG	cbc:CbuK_2095.
PATRIC	17919652. VBICoxBur77120_2101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_COXB1

Accession

Primary (citable) accession number: B6J6H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program