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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Coxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121MagnesiumUniRule annotation
Metal bindingi71 – 711MagnesiumUniRule annotation
Metal bindingi143 – 1431MagnesiumUniRule annotation
Metal bindingi146 – 1461MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCBUR434924:GHWU-1789-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:CbuK_1794
OrganismiCoxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154))
Taxonomic identifieri434924 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002504 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Crossover junction endodeoxyribonuclease RuvCPRO_1000090517Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi434924.CbuK_1794.

Structurei

3D structure databases

ProteinModelPortaliB6J504.
SMRiB6J504. Positions 7-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6J504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNPRRIIIG IDPGSRITGY GIIWSQGSKQ GCIAFGQIKT DNDSLNFRLH
60 70 80 90 100
QIERELRDLI LIHRPHEAAI EQVFTFHNHQ SALKLGQARG AALVATAACA
110 120 130 140 150
LPVAEYSARQ IKQAVVGYGA ATKAQVQHMV HLLLQLEKAP PADAADALAI
160 170
ALCHATSSRL SEKLMQAKGT LT
Length:172
Mass (Da):18,638
Last modified:December 15, 2008 - v1
Checksum:i2A4DED8B225AC0FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001020 Genomic DNA. Translation: ACJ20921.1.
RefSeqiWP_005772101.1. NC_011528.1.
YP_002306066.1. NC_011528.1.

Genome annotation databases

EnsemblBacteriaiACJ20921; ACJ20921; CbuK_1794.
GeneIDi7016307.
KEGGicbc:CbuK_1794.
PATRICi17919050. VBICoxBur77120_1806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001020 Genomic DNA. Translation: ACJ20921.1.
RefSeqiWP_005772101.1. NC_011528.1.
YP_002306066.1. NC_011528.1.

3D structure databases

ProteinModelPortaliB6J504.
SMRiB6J504. Positions 7-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi434924.CbuK_1794.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ20921; ACJ20921; CbuK_1794.
GeneIDi7016307.
KEGGicbc:CbuK_1794.
PATRICi17919050. VBICoxBur77120_1806.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciCBUR434924:GHWU-1789-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CbuK_Q154.

Entry informationi

Entry nameiRUVC_COXB1
AccessioniPrimary (citable) accession number: B6J504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: December 15, 2008
Last modified: February 3, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.