Reviewed, UniProtKB/Swiss-Prot B6J3R8 (METE_COXB2)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	CbuG_2053

Organism

Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)) [Complete proteome] [HAMAP]

Taxonomic identifier

434923 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 775	775	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097823
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6J3R8-1 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 94BE9061491891DE	FASTA	775	88,390
	10 20 30 40 50 60 MVYAHNLGFP RIGIKREMKK TVEAYWRGEI SQQQLQQQAI ELQLTNWKIQ AEAGVDLIPV 70 80 90 100 110 120 GDFSWYDHVL DMAVRVGAIP SRFKALNSNI TDTMFCTARG QAPNGIETSA CEMTKWFDTN 130 140 150 160 170 180 YHYIVPEFTT NQSFELHHDD LFKSTKLALE NNYRAKPVIL GPLSFLWLGK CKGESFNKLL 190 200 210 220 230 240 LLEKLLPVYA EIFEQLSSLG VEWVQVDEPI LVLDLPPEWQ QAFLTTYQQL NFFNLKCLLA 250 260 270 280 290 300 TYFGSLDDNL SLTCQLPVDG LHIDYCRAPD QLDSVLSQLP AEKILSVGIV DGRNIWCNDL 310 320 330 340 350 360 NRSLTLLENI QSSLGDRLWV APSCSLLHVP IDLDQENKLD VELKSWFAFA KQKVAEAAFL 370 380 390 400 410 420 TRGLREGRES IGAELKKNEE VIISRKTSKR IHNPNVEKKA ASVNERLMRR QHEHSIRKNK 430 440 450 460 470 480 QTAQLNLPLF PTTTIGSFPQ TSKIRCLRRD YKQGKIDDAL YEEKIRQEIA EVIGIQVKLG 490 500 510 520 530 540 LDVLVHGEPE RNDMVEYFGE LLDGIAITSN GWVQSYGSRC VKPPIIFGDV SRERPMTLRW 550 560 570 580 590 600 IEYAQSLTTK SVKGMLTGPV TILAWSFVRD DQPRSQTAKQ IALALRDEVQ DLERSGVRVI 610 620 630 640 650 660 QIDEPAFREC LPLRKAAWQD YLEWAVKCFR LASCGVKDET QIHTHMCYSE FNDIIEAIAA 670 680 690 700 710 720 LDADVITIES SRSEMEILKS FEKFAYPNDI GPGIYDIHSP RIPRVAEIEE LAVRALQYIP 730 740 750 760 770 IERLWINPDC GLKTRNWEET KEALSRMVDA AKHLRKAFSS EKTPTIDLEL QPAST

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001019 Genomic DNA. Translation: ACJ19290.1.
RefSeq	YP_002304435.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7014469.
GenomeReviews	Gene locus CbuG_2053 in contig CP001019_GR.
KEGG	cbg:CbuG_2053.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_COXB2

Accession

Primary (citable) accession number: B6J3R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)