B6J3C5 (B6J3C5_COXB2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACJ19250.1
EC=5.99.1.2 EMBL ACJ19250.1

Gene names

Name:	topA EMBL ACJ19250.1
Ordered Locus Names:	CbuG_2008

Organism

Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)) [Complete proteome] [HAMAP] EMBL ACJ19250.1

Taxonomic identifier

434923 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B6J3C5 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 82C6E4FB7AC2AA4C	FASTA	765	87,222
	10 20 30 40 50 60 MVKSLVVVES PAKAKTIEKY LGKDFKVMAS YGHVRDLIPK EGAVDPEDHF KMHYQAIERN 70 80 90 100 110 120 IRHMDAIAKA LKPCHNLYLA TDPDREGEAI AWHVKQMLES RNGLKDKKFS RVVFHQITKK 130 140 150 160 170 180 AVQHAIENPR DISMDLVNAQ QARRALDYLV GFNLSPLLWK KIRPGLSAGR VQSPALRLIV 190 200 210 220 230 240 EREIEIENFE SQEYWTIHAD CHAQQQPFDA RLIEFQGEKL EQFSIVNEKQ AHETRDAITQ 250 260 270 280 290 300 AANGKLAVAK VVKRERKRNP APPFITSTMQ QEAARKLGFS ASKTMQIAQQ LYEGVNLGEE 310 320 330 340 350 360 GAMGLITYMR TDSVTLASEA LQEIRQLIEE KYGQENVPEQ TRIYKTKSKN AQEAHEAIRP 370 380 390 400 410 420 TSAFRTPENL KKYLNSDQLK LYTLIWKRTI ACQMIEATLH TVAVDLKAPN AIFRANGSTI 430 440 450 460 470 480 VKPGFISVYQ ETFDDKKKEE ESDSKMLPPL EEGQTVDVNE IKSEQHFTEP PPRYTEASLV 490 500 510 520 530 540 KALEEYDIGR PSTYASIIAT LKNRKYVDVD NKRFIATDVG RIVNRFLTHY FTKYVDYGFT 550 560 570 580 590 600 AKLEDELDDI ARGEKTWIPV LETFWSPFKN LVDVTEKTVK RSDVTQEKID EKCPKCSAQL 610 620 630 640 650 660 SIRLGRRGRF IGCTAYPDCD YTRNLTETEA EAQAVLEGRQ CPECQSNLII KMGRYGKFIG 670 680 690 700 710 720 CSGYPNCKYM EPLEKPKDTN VTCPKCKKGT ILQRKSRRGK IFYSCARYPD CDYALWNEPV 730 740 750 760 AEACPKCGWP ILSIKTTKSR GTEKVCPQKS CNFIEAYDGD KPSLD

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001019 Genomic DNA. Translation: ACJ19250.1.
RefSeq	YP_002304395.1. NC_011527.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B6J3C5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7014424.
GenomeReviews	Gene locus CbuG_2008 in contig CP001019_GR.
KEGG	cbg:CbuG_2008.
PATRIC	17915140. VBICoxBur10955_2009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	IERNIRH.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6J3C5_COXB2

Accession

Primary (citable) accession number: B6J3C5

Entry history

Integrated into UniProtKB/TrEMBL:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)