B6J2S1 (TDH_COXB2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase
EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	CbuG_1900

Organism

Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)) [Complete proteome] [HAMAP]

Taxonomic identifier

434923 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00627
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity. HAMAP MF_00627
Subcellular location	Cytoplasm By similarity HAMAP MF_00627.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000130543
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6J2S1 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 71007C99C50DF6B6	FASTA	342	37,194
	10 20 30 40 50 60 MKVLSKLKPA PGLWLHKAPT PKPGRDEVLI KIKKTAICGT DLHIYKWDEW AQKTIPVPMH 70 80 90 100 110 120 VGHEFVGEIV EVGEAASALA VGDRVSGEGH ITCGDCRNCR AGKRHLCRYT VGVGVNRPGA 130 140 150 160 170 180 FAEYLVIPAK NAYKIPAKIS DDIAAILDPF GNAAHSALEF DLVGEDVLIT GAGPVGLMSA 190 200 210 220 230 240 AIARHVGARH VVITDVNDYR LALAEKVGVT AAVNSTKTPL TETMKNLGMT EGFDVGLEMS 250 260 270 280 290 300 GNAEAFRSML TVMNNGGKIA FLGIPPEPFA IDWNQVVFKS LLIKGIYGRR MFETWYKMTN 310 320 330 340 LLLSGLDISP IITHEFPMKD FQQAFDVMLS GKTGKVILNW EQ

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CbuG_Q212.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001019 Genomic DNA. Translation: ACJ19148.1.
RefSeq	YP_002304293.1. NC_011527.1.
3D structure databases
ProteinModelPortal	B6J2S1.
ModBase	Search...
Protein-protein interaction databases
STRING	B6J2S1.
Proteomic databases
PRIDE	B6J2S1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7014315.
GenomeReviews	Gene locus CbuG_1900 in contig CP001019_GR.
KEGG	cbg:CbuG_1900.
PATRIC	17914923. VBICoxBur10955_1903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753318.
OMA	MMRSGMS.
ProtClustDB	PRK05396.
Family and domain databases
HAMAP	MF_00627. Thr_dehydrog. [Tree]
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00060.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. Tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_COXB2

Accession

Primary (citable) accession number: B6J2S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program