B6J2A7 (SYGA_COXB2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	CbuG_0022

Organism

Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)) [Complete proteome] [HAMAP]

Taxonomic identifier

434923 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000101179

Sequences

	Sequence		Length	Mass (Da)
	B6J2A7 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: FF52579A9F0787F8	FASTA	319	36,397
	10 20 30 40 50 60 MKSSHPVNFQ QMILALQEYW ASQGCVLLQP FDMEVGAGTF HPATFLRAIG PEPWRAAYVQ 70 80 90 100 110 120 PSRRPTDGRY GDNPNRTQHY YQFQVVLKPS PDDIQDIYLG SLKALGIDPL THDIRFVEDN 130 140 150 160 170 180 WEAPTLGSWG VGWEVWQDGM EITQFTYFQQ IGGLECKPVT GEITYGLERL AMFLQGIDNM 190 200 210 220 230 240 FDLVWTEGPN GRVTYGQIFQ QNEVEMSAYN FEYANVEALF NFFDFYEKEA SQLIEVHLPL 250 260 270 280 290 300 AAYEMVLKAS HTFNLLDARQ AISVTERQRF ILRVRKLAQA VAEAYYSARE KLGFPMLEEI 310 SSSSSRVLPL AGNDRVKGC

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CbuG_Q212.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001019 Genomic DNA. Translation: ACJ17481.1.
RefSeq	YP_002302626.1. NC_011527.1.
3D structure databases
ProteinModelPortal	B6J2A7.
SMR	B6J2A7. Positions 9-285.
ModBase	Search...
Protein-protein interaction databases
STRING	B6J2A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7012439.
GenomeReviews	Gene locus CbuG_0022 in contig CP001019_GR.
KEGG	cbg:CbuG_0022.
PATRIC	17911049. VBICoxBur10955_0021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_COXB2

Accession

Primary (citable) accession number: B6J2A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program