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B6J1S2

- SYI_COXB2

UniProt

B6J1S2 - SYI_COXB2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CbuG_1612
Organism
Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi899 – 8991Zinc By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCBUR434923:GC8S-1609-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CbuG_1612
OrganismiCoxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Taxonomic identifieri434923 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000007729: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189143Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi434923.CbuG_1612.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B6J1S2-1 [UniParc]FASTAAdd to Basket

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MTDYKDTLNL PQTDFPMRAN LPEREPQTLA RWQTLDLYRK IRKDREGQPK    50
FILHDGPPYA NGRAHLGTAF NKTLKDIVVK SKTLSGFDAP FVPGWDCHGL 100
PIELNVEKKL GKDKLSANAF RQACRDYAFS QIELQRDDFQ RLGVLGDWQH 150
PYLTMDFGYE ADTVRALAKI VANGHLLRGQ KPVHWCAACG SALAEAEVEY 200
RDKASPAVDV GFEAVDAEAV RQRFGVKNAT TRVLVPIWTT TPWTLPANEA 250
VSVHPELHYA LVKSELQNQP VYLILAKDLV DSAMQRYGVD DYEVHGNLKG 300
DALEGMQLQH PFLDRIVPII LGEHVTTEAG TGNVHTAPAH GLEDYFVAEK 350
YNLPINNPVD ARGRFIPDTF LVGGQPVFKA NEPIIVLLAD SGHLLHSETI 400
QHSYPHCWRH KTPLIFRATP QWFIGMNKNG LRERALAEIE KVTWLPAWGE 450
ARIGKMVADR PDWCISRQRL WGIPIPLFIH KKSGELHPKS PALMEKVAQL 500
IEKESVDAWF DLDPKVLLGD DADHYEKVTD VLDVWFDSGV THFCVLEKRR 550
ELKVPADIYL EGSDQHRGWF QSSLLTSLAI RDKAPYKSVL TYGFVVDSQG 600
RKMSKSLGNV ILPADVVKNL GADVLRLWAA SMDYTVEVNV SDEILKRASD 650
AYRRIRNTAR FLLSNLYDFD PKKDKVAVDQ LVALDRWAIF TTQKLQEKII 700
TAYDRYRFPA IYQAIHNFCT VEMGSFYLDI IKDRLYTSKE SGLPRRSAQT 750
ALYYIAEAFV RWIAPIISFT ADEIWQFMPG DREPSVFLTQ WFSDFPNAAL 800
SGEEEQRWQL LLQIRDEVNK ALETYRNEGK IGSALTAEVV LYADERLNAA 850
IATLGEELRF VLITSEASVL PFNEKSKAAF DTALPGLALE INVSEFEKCA 900
RCWQRRSSVG QIKEHADLCD RCVSNAFEDG DMRQFA 936
Length:936
Mass (Da):106,126
Last modified:December 16, 2008 - v1
Checksum:i11B778F5F7F9C450
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001019 Genomic DNA. Translation: ACJ18900.1.
RefSeqiWP_012570344.1. NC_011527.1.
YP_002304045.1. NC_011527.1.

Genome annotation databases

EnsemblBacteriaiACJ18900; ACJ18900; CbuG_1612.
GeneIDi7014028.
KEGGicbg:CbuG_1612.
PATRICi17914305. VBICoxBur10955_1611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001019 Genomic DNA. Translation: ACJ18900.1 .
RefSeqi WP_012570344.1. NC_011527.1.
YP_002304045.1. NC_011527.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 434923.CbuG_1612.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ18900 ; ACJ18900 ; CbuG_1612 .
GeneIDi 7014028.
KEGGi cbg:CbuG_1612.
PATRICi 17914305. VBICoxBur10955_1611.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CBUR434923:GC8S-1609-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CbuG_Q212.

Entry informationi

Entry nameiSYI_COXB2
AccessioniPrimary (citable) accession number: B6J1S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 16, 2008
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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