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B6J1B2 (SYL_COXB2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CbuG_1438
OrganismCoxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)) [Complete proteome] [HAMAP]
Taxonomic identifier434923 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 820820Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091310

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B6J1B2 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 06B683979B235DCF

FASTA82094,273
        10         20         30         40         50         60 
MNESYQPTLI EQLAQEYWEE NETFEVKEDL SREKFYCLSM LPYPSGDLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VIARYQIHKG RNVLQPMGWD AFGLPAENAA IQRELPPAEW TRKNIKKMRK QLKQLGFAYD 

       130        140        150        160        170        180 
WSREITTCDS TYYRWEQWLF LQLYKKGLAY KKNAIVNWDP VDQTVLANEQ IVDGRGWRSG 

       190        200        210        220        230        240 
AVVERREISQ WFLKITDYSE ELLKDLDELK EWPEQVITMQ RNWIGQSQGV IINFNLEKGP 

       250        260        270        280        290        300 
DKLQVYTTRP DTLMGVTYLA IAPEHPLAKE RAKKSKKIAA FLKKCKQTRV AEADIATQEK 

       310        320        330        340        350        360 
EGIDSGLFAV HPLSKEKLPI WIANFVLMEY ASGVVMAVPA HDERDHEFAL KYDLPLKPVI 

       370        380        390        400        410        420 
EPADGHDWDY NQAAYTNPGK LINSGSFNDI DSKTAFNLIA DYLKNNGAGS RQTHYRLRDW 

       430        440        450        460        470        480 
GISRQRYWGT PIPIIYCKTC GTVPVPENQL PVLLPEDIIP TGHGSPLKET ASFYKTRCPV 

       490        500        510        520        530        540 
CNKPATRETD TMDTFVESSW YYARYSCPDQ DKVMLDDRAK YWTPVDQYIG GIEHAVMHLL 

       550        560        570        580        590        600 
YARFMHKILR DLGLLNSNEP FIRLLTQGMV LKDGAKMSKS KGNVVTPQSL IKKYGADTVR 

       610        620        630        640        650        660 
LFIIFAAPPE QDLEWSDSGV EGAYRFLKKL WGFSYRIKDA LLAINQQKER SNYQWEAPEH 

       670        680        690        700        710        720 
RQTRQQIHEC LQQANIDMER LQFNTVVSAV MKILNILIKL TTDNDAEAHL IREGTGILLR 

       730        740        750        760        770        780 
LLSPITPHIS HHLWQSLGFG GDILDTPWPR PDPKALQTTE LELIVQINGK LRGRIQVPTE 

       790        800        810        820 
ASKEIIESTA LNQENVQRHL ADKKIKKVIV VPKKLINIVV 

« Hide

References

[1]"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CbuG_Q212.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001019 Genomic DNA. Translation: ACJ18740.1.
RefSeqYP_002303885.1. NC_011527.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434923.CbuG_1438.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ18740; ACJ18740; CbuG_1438.
GeneID7013854.
KEGGcbg:CbuG_1438.
PATRIC17913961. VBICoxBur10955_1441.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCBUR434923:GC8S-1436-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_COXB2
AccessionPrimary (citable) accession number: B6J1B2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries