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B6J074

- BIOB_COXB2

UniProt

B6J074 - BIOB_COXB2

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Protein
Biotin synthase
Gene
bioB, CbuG_1000
Organism
Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi127 – 1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi187 – 1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi259 – 2591Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBUR434923:GC8S-998-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CbuG_1000
OrganismiCoxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Taxonomic identifieri434923 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000007729: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Biotin synthaseUniRule annotation
PRO_0000381332Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi434923.CbuG_1000.

Structurei

3D structure databases

ProteinModelPortaliB6J074.
SMRiB6J074. Positions 4-310.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B6J074-1 [UniParc]FASTAAdd to Basket

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MKGRNWNQAS VAKLFELPFF ELLYKAYETH RSHFDVRDME LCTLSSIKTG    50
TCPEDCAYCP QSGHYKTDVE REKLINLEAV LEQAKVAKEN GARRFCMGAA 100
WRSPPKRELP KVLEMIKSVK ALGLETCVTL GMLDQEQALQ LKEAGLDFYN 150
HNLDTSPEFY KKIITTRTYQ DRMETLKNVR NAGINVCCGG ILGMGESRAD 200
RIQLLLELYQ LPEPPTSIPI NQLIPIKGTP LENTKAIDPF EFIKTIAITR 250
LLFPTSVIRL SAGREAMSDE LQAWCFMAGA NSIFYGDKLL TAKNPGQNRD 300
VNLLKKLGLK VPVLTEEYAC Y 321
Length:321
Mass (Da):36,252
Last modified:December 16, 2008 - v1
Checksum:iFDC79146CEC713D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001019 Genomic DNA. Translation: ACJ18352.1.
RefSeqiYP_002303497.1. NC_011527.1.

Genome annotation databases

EnsemblBacteriaiACJ18352; ACJ18352; CbuG_1000.
GeneIDi7013416.
KEGGicbg:CbuG_1000.
PATRICi17913041. VBICoxBur10955_0995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001019 Genomic DNA. Translation: ACJ18352.1 .
RefSeqi YP_002303497.1. NC_011527.1.

3D structure databases

ProteinModelPortali B6J074.
SMRi B6J074. Positions 4-310.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434923.CbuG_1000.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ18352 ; ACJ18352 ; CbuG_1000 .
GeneIDi 7013416.
KEGGi cbg:CbuG_1000.
PATRICi 17913041. VBICoxBur10955_0995.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CBUR434923:GC8S-998-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CbuG_Q212.

Entry informationi

Entry nameiBIOB_COXB2
AccessioniPrimary (citable) accession number: B6J074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 16, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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