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B6IZL4

- LIPA_COXB2

UniProt

B6IZL4 - LIPA_COXB2

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Protein

Lipoyl synthase

Gene

lipA

Organism
Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBUR434923:GC8S-743-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:CbuG_0745
OrganismiCoxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Taxonomic identifieri434923 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000007729: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Lipoyl synthasePRO_1000099598Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi434923.CbuG_0745.

Structurei

3D structure databases

ProteinModelPortaliB6IZL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B6IZL4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTYDPSQKS LGKEKLSRIP VKIEATHTPL RKPDWIRIRL STDSKVSQLK
60 70 80 90 100
KLLRENHLVT VCEEASCPNL NECFGHGTAT FMIMGDKCTR RCSFCDVGHG
110 120 130 140 150
RPDPLDPEEP VNLANTVSIM SLRYVVITSV DRDDLRDGGA QHYAQCINAV
160 170 180 190 200
REKNPGIKVE VLVPDFRGRM EKALDQLAQG LPDVFNHNIE TAPRLYKQAR
210 220 230 240 250
PGADYPWSLA LLQTFKKRFP GIPTKSGMML GLGETREEVE MVMRDLRQHE
260 270 280 290 300
VDRLTLGQYL QPTRYHMPVD RYVTPQEFQE LGELAKKLGF SNVASGPLVR
310
SSYHADLQAQ GERVS
Length:315
Mass (Da):35,604
Last modified:December 16, 2008 - v1
Checksum:i9DC26837BAD4EF80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001019 Genomic DNA. Translation: ACJ18142.1.
RefSeqiWP_010958112.1. NC_011527.1.
YP_002303287.1. NC_011527.1.

Genome annotation databases

EnsemblBacteriaiACJ18142; ACJ18142; CbuG_0745.
GeneIDi7013161.
KEGGicbg:CbuG_0745.
PATRICi17912483. VBICoxBur10955_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001019 Genomic DNA. Translation: ACJ18142.1 .
RefSeqi WP_010958112.1. NC_011527.1.
YP_002303287.1. NC_011527.1.

3D structure databases

ProteinModelPortali B6IZL4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434923.CbuG_0745.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ18142 ; ACJ18142 ; CbuG_0745 .
GeneIDi 7013161.
KEGGi cbg:CbuG_0745.
PATRICi 17912483. VBICoxBur10955_0726.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CBUR434923:GC8S-743-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CbuG_Q212.

Entry informationi

Entry nameiLIPA_COXB2
AccessioniPrimary (citable) accession number: B6IZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3