B6ISU1 (LPXD_RHOCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	RC1_1198

Organism

Rhodospirillum centenum (strain ATCC 51521 / SW) [Complete proteome] [HAMAP]

Taxonomic identifier

414684 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127695
Sites
	Active site	251	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ISU1 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: AE3A33E6B4180A30	FASTA	347	35,407
	10 20 30 40 50 60 MADPRFFRRA GPFSLAQLAS LSGAEIAPGA DPALLLHDVA PLESAGPEQL SFLDNRKYAD 70 80 90 100 110 120 AFARTGAGAC IVHPDLAGRA PAGTALLLSR KPYRAYALCA QAFYPAPAAE GGVSPGAHLH 130 140 150 160 170 180 PTARVGEGTE VAPGAVIEAG AEIGNGCRIG PNAVIGRNVR IGDGTTVGAC ASLSHCEIGS 190 200 210 220 230 240 RVVIYPGVRI GQDGFGFAMD VAGHVRVPQL GRVLVEDDVE IGANVTIDRG AGPDTVIGRG 250 260 270 280 290 300 CMIDNLVQIG HNVHLGPGCV VVAQAGISGS TKLDHHVILA AQAGITGHLK IGAGARIAAQ 310 320 330 340 SGVMRDVAPG EQVGGSPAVP MRQWLRQVAV LGRLVRGRGD EDNGRNG

References

[1]	"Genome sequence of Rhodospirillum centenum." Touchman J.W., Bauer C., Blankenship R.E. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51521 / SW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000613 Genomic DNA. Translation: ACI98612.1.
RefSeq	YP_002297425.1. NC_011420.2.
3D structure databases
ProteinModelPortal	B6ISU1.
ModBase	Search...
Protein-protein interaction databases
STRING	B6ISU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7005968.
GenomeReviews	Gene locus RC1_1198 in contig CP000613_GR.
KEGG	rce:RC1_1198.
PATRIC	23317604. VBIRhoCen1465_1153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	VQIGRVI.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 2 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RHOCS

Accession

Primary (citable) accession number: B6ISU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program