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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Rhodospirillum centenum (strain ATCC 51521 / SW)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Charge relay systemUniRule annotation
Active sitei189 – 1891Charge relay systemUniRule annotation
Active sitei213 – 2131Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRCEN414684:GHCM-1497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:RC1_1516
OrganismiRhodospirillum centenum (strain ATCC 51521 / SW)
Taxonomic identifieri414684 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
Proteomesi
  • UP000001591 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000095165Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi414684.RC1_1516.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 2 hits.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 2 hits.
PfamiPF01425. Amidase. 2 hits.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 2 hits.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6IN23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSLNHLTMA EALAGLRAGE FTATELTEAH LRAVEATRGL NIFITETADI
60 70 80 90 100
ALRQAKAADE RYAAGTALPM DGLPIAVKDL FCTEGVLTTA ASHILDGFKP
110 120 130 140 150
PYESSVTANL WRDGAVMLGK VNLDEFAMGS ANITSYYGPV ISPWSKGRRV
160 170 180 190 200
QRDAAPAGSG VLSEVAEAFR QPQPVTEWEW ERRLVPGGSS GGSAAAVAAR
210 220 230 240 250
VAMAATGTDT GGSIRQPASF VGIVGLKPTY GRCSRWGIVA FASSLDQAGP
260 270 280 290 300
MTRTVRDAAI MLRSMAGFDP KDSTSADVPV PDYEKAITGD IRGLRVGIPR
310 320 330 340 350
EYRVDGMPAE IDRLWQQGVE WLKAAGAVPV EISLPHTKYA LPAYYIVAPA
360 370 380 390 400
EASSNLARYD GVRFGLRVEG KDLKEMYENT RGEGFGREVR RRILIGTYVL
410 420 430 440 450
SAGYYDAYYL KAQKVRARIA EDFTKAWEQC DVILTPTAPN TAFGIGEKMD
460 470 480 490 500
DPIAMYLNDV FTVPANLAGL PGLSVPAGLA ANGLPLGLQL VGRPFDEATL
510 520
IRTAAVLEQA AGPQPLPPVH A
Length:521
Mass (Da):55,749
Last modified:December 16, 2008 - v1
Checksum:iC2999B0DD5D674D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000613 Genomic DNA. Translation: ACI98920.1.
RefSeqiWP_012566706.1. NC_011420.2.

Genome annotation databases

EnsemblBacteriaiACI98920; ACI98920; RC1_1516.
KEGGirce:RC1_1516.
PATRICi23318228. VBIRhoCen1465_1459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000613 Genomic DNA. Translation: ACI98920.1.
RefSeqiWP_012566706.1. NC_011420.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi414684.RC1_1516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI98920; ACI98920; RC1_1516.
KEGGirce:RC1_1516.
PATRICi23318228. VBIRhoCen1465_1459.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Enzyme and pathway databases

BioCyciRCEN414684:GHCM-1497-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 2 hits.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 2 hits.
PfamiPF01425. Amidase. 2 hits.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 2 hits.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_RHOCS
AccessioniPrimary (citable) accession number: B6IN23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: September 7, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.