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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Escherichia coli (strain SE11)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length.UniRule annotation

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.UniRule annotation
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.UniRule annotation
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].UniRule annotation

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseUniRule annotation (EC:4.2.1.59UniRule annotation)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabAUniRule annotation
Beta-hydroxydecanoyl thioester dehydraseUniRule annotation
Trans-2-decenoyl-[acyl-carrier-protein] isomeraseUniRule annotation (EC:5.3.3.14UniRule annotation)
Gene namesi
Name:fabAUniRule annotation
Ordered Locus Names:ECSE_1015
OrganismiEscherichia coli (strain SE11)
Taxonomic identifieri409438 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002011791 – 1723-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseAdd BLAST172

Proteomic databases

PRIDEiB6I928.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB6I928.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thioester dehydratase family. FabA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.

Family and domain databases

CDDicd01287. FabA. 1 hit.
Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA. 1 hit.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

B6I928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDKRESYTK EDLLASGRGE LFGTKGPQLP APNMLMMDRV VKMTETGGNF
60 70 80 90 100
DKGYVEAELD INPDLWFFGC HFIGDPVMPG CLGLDAMWQL VGFYLGWLGG
110 120 130 140 150
EGKGRALGVG EVKFTGQVLP TAKKVTYRIH FKRIVNRRLI MGLADGEVLV
160 170
DGRLIYTASD LKVGLFQDTS AF
Length:172
Mass (Da):18,999
Last modified:December 16, 2008 - v1
Checksum:i2AC51C64677D212D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009240 Genomic DNA. Translation: BAG76539.1.
RefSeqiWP_001609763.1. NC_011415.1.

Genome annotation databases

EnsemblBacteriaiBAG76539; BAG76539; ECSE_1015.
KEGGiecy:ECSE_1015.
PATRICi18420492. VBIEscCol83070_1147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009240 Genomic DNA. Translation: BAG76539.1.
RefSeqiWP_001609763.1. NC_011415.1.

3D structure databases

ProteinModelPortaliB6I928.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB6I928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG76539; BAG76539; ECSE_1015.
KEGGiecy:ECSE_1015.
PATRICi18420492. VBIEscCol83070_1147.

Phylogenomic databases

HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

CDDicd01287. FabA. 1 hit.
Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA. 1 hit.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABA_ECOSE
AccessioniPrimary (citable) accession number: B6I928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.