B6I385 (TREF_ECOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytoplasmic trehalase
EC=3.2.1.28

Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Gene names

Name:	treF
Ordered Locus Names:	ECSE_3788

Organism

Escherichia coli (strain SE11) [Complete proteome] [HAMAP]

Taxonomic identifier

409438 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity By similarity. HAMAP MF_01059
Catalytic activity	Alpha,alpha-trehalose + H2O = 2 D-glucose. HAMAP MF_01059
Pathway	Glycan degradation; trehalose degradation; D-glucose from alpha,alpha-trehalose: step 1/1. HAMAP MF_01059
Subunit structure	Monomer By similarity. HAMAP MF_01059
Subcellular location	Cytoplasm By similarity HAMAP MF_01059.
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular hyperosmotic response Inferred from electronic annotation. Source: InterPro trehalose catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha,alpha-trehalase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Cytoplasmic trehalase HAMAP MF_01059		PRO_1000136405

Sequences

	Sequence		Length	Mass (Da)
	B6I385 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 543B83F6BFC1CB9D	FASTA	549	63,715
	10 20 30 40 50 60 MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS DALTPADRYL 70 80 90 100 110 120 ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH RDFDLRKFVE NHFWLPEVYS 130 140 150 160 170 180 SEYVSDPQNS LKEHIDQLWP VLTREPQDHI PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT 190 200 210 220 230 240 MLGLAESGRE DLLKCMADNF AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR 250 260 270 280 290 300 GARRYLDHLK MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV 310 320 330 340 350 360 ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID LNAFLFKLES 370 380 390 400 410 420 AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI YRDYDWRREQ LALFSAAAIV 430 440 450 460 470 480 PLYVGMANHE QADRLANAVR SRLLTPGGIL ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY 490 500 510 520 530 540 GDDLLGDEIA RSWLKTVNQF YLEQHKMIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR RLIGLYGEP

References

[1]

"Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult." Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., Ooka T., Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M. DNA Res. 15:375-386(2008) [PubMed: 18931093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SE11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009240 Genomic DNA. Translation: BAG79312.1.
RefSeq	YP_002295063.1. NC_011415.1.
3D structure databases
ProteinModelPortal	B6I385.
SMR	B6I385. Positions 53-548.
ModBase	Search...
Protein-protein interaction databases
STRING	B6I385.
Protein family/group databases
CAZy	GH37. Glycoside Hydrolase Family 37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000110649; EBESCP00000102607; EBESCG00000105426.
GeneID	7002173.
GenomeReviews	Gene locus ECSE_3788 in contig AP009240_GR.
KEGG	ecy:ECSE_3788.
PATRIC	18426219. VBIEscCol83070_3962.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010574.
HOGENOM	HBG485982.
OMA	FWMDGAD.
ProtClustDB	PRK13270.
Enzyme and pathway databases
BioCyc	ECOL409438:ECSE_3788-MONOMER.
Family and domain databases
HAMAP	MF_01059. Cyt_trehalase. [Tree]
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR023715. Cyt_trehalase. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. [Graphical view]
KO	K01194.
PANTHER	PTHR23403. Glyco_hydro_37. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TREF_ECOSE

Accession

Primary (citable) accession number: B6I385

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program