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B6I1P3 (IF2_ECOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:ECSE_3454
OrganismEscherichia coli (strain SE11) [Complete proteome] [HAMAP]
Taxonomic identifier409438 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   PTMAcetylation
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000093784

Regions

Nucleotide binding398 – 4058GTP By similarity
Nucleotide binding444 – 4485GTP By similarity
Nucleotide binding498 – 5014GTP By similarity
Region392 – 540149G-domain HAMAP-Rule MF_00100

Amino acid modifications

Modified residue8081N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B6I1P3 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 86B9F9B2AE773DDE

FASTA89097,350
        10         20         30         40         50         60 
MTDVTIKTLA AERQTSVERL VQQFADAGIR KSADDSVSAQ EKQTLIDHLN QKNSGPDKLT 

        70         80         90        100        110        120 
LQRKTRSTLN IPGTGGKSKS VQIEVRKKRT FVKRDPQEAE RLAAEEQAQR EAEEQARREA 

       130        140        150        160        170        180 
EESAKREAQQ KAEREAAEQA KREAAEQAKR EAAEKDKVSN QQDDMTKNAQ AEKARREQEA 

       190        200        210        220        230        240 
AELKRKAEEE ARRKLEEEAR RVAEEARRMA EENKWTDNAE PTEDSSDYHV TTSQHARQAE 

       250        260        270        280        290        300 
DESDREVEGG RGRGRNAKAA RPKKGNKHAE SKADREEARA AVRGGKGGKR KGSSLQQGFQ 

       310        320        330        340        350        360 
KPAQAVNRDV VIGETITVGE LANKMAVKGS QVIKAMMKLG AMATINQVID QETAQLVAEE 

       370        380        390        400        410        420 
MGHKVILRRE NELEEAVMSD RDTGAAAEPR APVVTIMGHV DHGKTSLLDY IRSTKVASGE 

       430        440        450        460        470        480 
AGGITQHIGA YHVETENGMI TFLDTPGHAA FTSMRARGAQ ATDIVVLVVA ADDGVMPQTI 

       490        500        510        520        530        540 
EAIQHAKAAQ VPVVVAVNKI DKPEADPDRV KNELSQYGIL PEEWGGESQF VHVSAKAGTG 

       550        560        570        580        590        600 
IDELLDAILL QAEVLELKAV RKGMASGAVI ESFLDKGRGP VATVLVREGT LHKGDIVLCG 

       610        620        630        640        650        660 
FEYGRVRAMR NELGQEVLEA GPSIPVEILG LSGVPAAGDE VTVVRDEKKA REVALYRQGK 

       670        680        690        700        710        720 
FREVKLARQQ KSKLENMFAN MTEGEVHEVN IVLKADVQGS VEAISDSLLK LSTDEVKVKI 

       730        740        750        760        770        780 
IGSGVGGITE TDATLAAASN AILVGFNVRA DASARKVIEA ESLDLRYYSV IYNLIDEVKA 

       790        800        810        820        830        840 
AMSGMLSPEL KQQIIGLAEV RDVFKSPKFG AIAGCMVTEG VVKRHNPIRV LRDNVVIYEG 

       850        860        870        880        890 
ELESLRRFKD DVNEVRNGME CGIGVKNYND VRTGDVIEVF EIIEIQRTIA 

« Hide

References

[1]"Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult."
Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., Ooka T., Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.
DNA Res. 15:375-386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SE11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009240 Genomic DNA. Translation: BAG78978.1.
RefSeqYP_002294729.1. NC_011415.1.

3D structure databases

ProteinModelPortalB6I1P3.
SMRB6I1P3. Positions 2-50, 316-887.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING409438.ECSE_3454.

Proteomic databases

PRIDEB6I1P3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG78978; BAG78978; ECSE_3454.
GeneID7001839.
KEGGecy:ECSE_3454.
PATRIC18425522. VBIEscCol83070_3623.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMAHDERGHE.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycECOL409438:GHUU-3512-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_ECOSE
AccessionPrimary (citable) accession number: B6I1P3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries