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Protein

Nitrilase

Gene

Pc22g19330ma2m

Organism
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that hydrolyzes preferentially 4-cyanopyridine. Is also able to hydrolyze some aliphatic nitriles, such as (R,S)-mandelonitrile.1 Publication

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Proton acceptor1
Active sitei1311
Active sitei166Nucleophile1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciPCHR:PC22G19330-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase1 Publication (EC:3.5.5.11 Publication)
Gene namesi
ORF Names:Pc22g19330ma2m
OrganismiPenicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Taxonomic identifieri500485 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum species complex
Proteomesi
  • UP000000724 Componenti: Contig Pc00c22

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004321801 – 328NitrilaseAdd BLAST328

Interactioni

Protein-protein interaction databases

STRINGi500485.XP_002565836.1.

Structurei

3D structure databases

ProteinModelPortaliB6HVR6.
SMRiB6HVR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 286CN hydrolaseAdd BLAST278

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
HOGENOMiHOG000256364.
OMAiYSERDGD.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B6HVR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPVERRVR VAAIQAEPVW NDLQGGVNKV ISLLGDVGKE GANVVGFPEV
60 70 80 90 100
FIPGYPWSIF TATPLDNAPF MEEYFHNSLA VDSDEMRRIQ AAVKENGTFC
110 120 130 140 150
VLGFSERYQG SLYISQVFIN TDGQIVHHRR KTKPTHVERA YWGTGEGDSL
160 170 180 190 200
KCVVDSPFGR IGGLNCWEHT QPLLRYYEYQ QDVDIHVASW PVLWDRPESV
210 220 230 240 250
GSRWPYFITG DMSSRLSQVM AFEGTCFVLV CTQVMSEENF DKNKVRDVEH
260 270 280 290 300
IQGTGGGFSA IFGPGGEPIA TMPSDKEGIL YANVDVNDKL RAKQWLDVVG
310 320
HYSRPDLLSL RVNTHPSKPV FFAEEPEK
Length:328
Mass (Da):36,809
Last modified:December 16, 2008 - v1
Checksum:iAA68D3B2A6F1AD61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920437 Genomic DNA. Translation: CAP99221.1.
RefSeqiXP_002565836.1. XM_002565790.1.

Genome annotation databases

EnsemblFungiiCAP99221; CAP99221; PCH_Pc22g19330.
GeneIDi8315936.
KEGGipcs:Pc22g19330.

Similar proteinsi

Entry informationi

Entry nameiNIT2_PENRW
AccessioniPrimary (citable) accession number: B6HVR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: December 16, 2008
Last modified: October 25, 2017
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families