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Protein

Cyanide hydratase

Gene

PCH_Pc18g02620

Organism
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydration of cyanide to formamide. Degradation of cyanide may be important for plant pathogenic fungi in infection of cyanogenic plants.UniRule annotation1 Publication

Catalytic activityi

Formamide = cyanide + H2O.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48Proton acceptorUniRule annotation1
Active sitei130UniRule annotation1
Active sitei165NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase

Enzyme and pathway databases

BioCyciPCHR:PC18G02620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyanide hydratase1 PublicationUniRule annotation (EC:4.2.1.66UniRule annotation1 Publication)
Short name:
CHTUniRule annotation
Alternative name(s):
Cyanide-degrading nitrilaseUniRule annotation
Formamide hydrolyaseUniRule annotation
Gene namesi
ORF Names:PCH_Pc18g02620
OrganismiPenicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Taxonomic identifieri500485 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum species complex
Proteomesi
  • UP000000724 Componenti: Contig Pc00c18

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004321811 – 358Cyanide hydrataseAdd BLAST358

Expressioni

Inductioni

By cyanide.UniRule annotation

Interactioni

Subunit structurei

Oligomer of dimers, forming left-handed helical fibers.UniRule annotation

Protein-protein interaction databases

STRINGi500485.XP_002562104.1.

Structurei

3D structure databases

ProteinModelPortaliB6HCY4.
SMRiB6HCY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 287CN hydrolasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
HOGENOMiHOG000256364.
KOiK10675.
OMAiNGHARIY.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
HAMAPiMF_03224. CN_hydrolase. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.

Sequencei

Sequence statusi: Complete.

B6HCY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPVLKKYKA AAVNAEPGWF DLQESVRRTI HWIDEAGKAG CKLIAFPELW
60 70 80 90 100
IPGYPYWAWK VNYQESLPLL KKYRENSLPS DSDEMRRIRE AAKANKIWVS
110 120 130 140 150
LGYSELDLAS LYTTQIMISP AGDVINHRRK IKATHVERLV FGDGTGDTTE
160 170 180 190 200
SVMDTEIGRI GHLNCWENMN PFLKAYAASL GEQVHIAAWP LYPGKETLKY
210 220 230 240 250
PDPYTNVAEA NADLVTPAYA IETGSFTLAP WQTITAEGIK LNTPPGKELE
260 270 280 290 300
DPNIYNGNGR IFGPDGQNLV PHPDKDFQGL LFVDIDLDEI HLTKSLADFG
310 320 330 340 350
GHYMRPDLIR LLVDTNRKDL VVHEDRVNGG VAYTRTIDRV GLSAPLDASA

TEAQSESV
Length:358
Mass (Da):39,966
Last modified:December 16, 2008 - v1
Checksum:i7BCD54631BEBDC2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920433 Genomic DNA. Translation: CAP94486.1.
RefSeqiXP_002562104.1. XM_002562058.1.

Genome annotation databases

EnsemblFungiiCAP94486; CAP94486; PCH_Pc18g02620.
GeneIDi8316291.
KEGGipcs:Pc18g02620.

Similar proteinsi

Entry informationi

Entry nameiCHT_PENRW
AccessioniPrimary (citable) accession number: B6HCY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: December 16, 2008
Last modified: October 25, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families