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B6GW04

- BGALB_PENCW

UniProt

B6GW04 - BGALB_PENCW

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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Penicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei196 – 1961Proton donorSequence Analysis
Binding sitei265 – 2651SubstrateBy similarity
Active sitei308 – 3081NucleophileSequence Analysis
Binding sitei373 – 3731SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BioCyciPCHR:PC06G00600-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:Pc06g00600
OrganismiPenicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum)
Taxonomic identifieri500485 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum complex
ProteomesiUP000000724: Contig Pc00c06

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 1013992Probable beta-galactosidase BPRO_5000408640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi768 – 7681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi500485.B6GW04.

Structurei

3D structure databases

ProteinModelPortaliB6GW04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B6GW04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRILNCLLV LLACLGVSSK AEDQAVTQWP LQDNGLNTVV QWDHYSFQIN
60 70 80 90 100
GQRIFIFSGE FHYWRIPVPA LWRDILEKIK AAGFTAFAFY SSWAYHAPNN
110 120 130 140 150
ATVDFTTGAR DITPIFELAK ELGMYIIVRP GPYVNAEANA GGFPLWVTTG
160 170 180 190 200
DYGTLRNDDT RYTNAWTPYF TEVTEITSRY QVTDGHYSIV YQIENEYGNQ
210 220 230 240 250
WLGDPTLRVP NETAIAYMEL LKANARDNGI TLPLTVNDPN MKTHSWGKDW
260 270 280 290 300
SDAGGNVDVA GLDSYPSCWT CDISQCTSTN GAYVPFQVLE YHDYFQESQP
310 320 330 340 350
SMPAFMPEFQ GGSYNPWGGP EGGCPGDIGD DFANLFYRWN IGQRVTAMSL
360 370 380 390 400
YMMFGGQNPG AMAAPVTASS YDYSAPISED RSIWSKYHET KLLALFTRSA
410 420 430 440 450
KDLTMTELMG NGTQYTDNPA VRAYELRNPE TNSAFYATFH SNTSISTNEP
460 470 480 490 500
FHLKVNTSAG VLTVPKYAST IRLNGHQSKI IVTDFTFGSK SLLYSTAEVL
510 520 530 540 550
TYAVFDKKPT LVLWVPTGES GEFSIKGAKK GSIKKCQGCS RVKFIKEHGG
560 570 580 590 600
LTTSLTQSAG MTVLEFDDGV RVILLDRTSA YDFWAPALTN DPFVPETESV
610 620 630 640 650
LIQGPYLVRD AKLSGSKLAI TGDVVNATTL DVFAPKGVKS VTWNGKKVDT
660 670 680 690 700
HSTEYGSLKG SLDAPQSIKL PALASWKSKD SLPERFADYD DSGAAWVDAN
710 720 730 740 750
HMTTLNPRTP TSLPVLYADQ YGFHNGVRLW RGYFNGTATG AFINVQGGSA
760 770 780 790 800
FGWSAWLNGE FLASHLGNAT TSQANLSLSF TDATLHTDTP NVLLIVHDDT
810 820 830 840 850
GHDQTTGALN PRGIMDAKLL GSDSGFTHWR LAGTAGGESD LDPVRGVYNE
860 870 880 890 900
DGLFAERVGW HLPGFDDSDW GEEGSAKDST TSVLSFEGAT VRFFRTTCPL
910 920 930 940 950
DIPAHTDVSI SFVLSTPAGA TTEYRAQLFV NGYQYGRYNP YIGNQVVYPV
960 970 980 990 1000
PVGILDYKGE NTIGVAVWAQ SEEGASIGID WRVNYLADSS LDVASWDTKD
1010
LRPGWTEERV KYA
Length:1,013
Mass (Da):111,429
Last modified:July 13, 2010 - v2
Checksum:iA4858AD5E5C13BB2
GO

Sequence cautioni

The sequence CAP79053.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920421 Genomic DNA. Translation: CAP79053.1. Sequence problems.
RefSeqiXP_002556674.1. XM_002556628.1.

Genome annotation databases

GeneIDi8315677.
KEGGipcs:Pc06g00600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920421 Genomic DNA. Translation: CAP79053.1 . Sequence problems.
RefSeqi XP_002556674.1. XM_002556628.1.

3D structure databases

ProteinModelPortali B6GW04.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 500485.B6GW04.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8315677.
KEGGi pcs:Pc06g00600.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
OrthoDBi EOG7ZGXBD.

Enzyme and pathway databases

BioCyci PCHR:PC06G00600-MONOMER.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SMARTi SM01029. BetaGal_dom2. 1 hit.
[Graphical view ]
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 28089 / DSM 1075 / Wisconsin 54-1255.

Entry informationi

Entry nameiBGALB_PENCW
AccessioniPrimary (citable) accession number: B6GW04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: October 29, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3