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B6GW04

- BGALB_PENCW

UniProt

B6GW04 - BGALB_PENCW

Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Penicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 2 (13 Jul 2010)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei135 – 1351SubstrateBy similarity
    Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei195 – 1951SubstrateBy similarity
    Active sitei196 – 1961Proton donorSequence Analysis
    Binding sitei265 – 2651SubstrateBy similarity
    Active sitei308 – 3081NucleophileSequence Analysis
    Binding sitei373 – 3731SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciPCHR:PC06G00600-MONOMER.

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase B (EC:3.2.1.23)
    Alternative name(s):
    Lactase B
    Gene namesi
    Name:lacB
    ORF Names:Pc06g00600
    OrganismiPenicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum)
    Taxonomic identifieri500485 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum complex
    ProteomesiUP000000724: Contig Pc00c06

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 1013992Probable beta-galactosidase BPRO_5000408640Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi271 ↔ 324By similarity
    Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi768 – 7681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi500485.B6GW04.

    Structurei

    3D structure databases

    ProteinModelPortaliB6GW04.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B6GW04-1 [UniParc]FASTAAdd to Basket

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    MTRILNCLLV LLACLGVSSK AEDQAVTQWP LQDNGLNTVV QWDHYSFQIN     50
    GQRIFIFSGE FHYWRIPVPA LWRDILEKIK AAGFTAFAFY SSWAYHAPNN 100
    ATVDFTTGAR DITPIFELAK ELGMYIIVRP GPYVNAEANA GGFPLWVTTG 150
    DYGTLRNDDT RYTNAWTPYF TEVTEITSRY QVTDGHYSIV YQIENEYGNQ 200
    WLGDPTLRVP NETAIAYMEL LKANARDNGI TLPLTVNDPN MKTHSWGKDW 250
    SDAGGNVDVA GLDSYPSCWT CDISQCTSTN GAYVPFQVLE YHDYFQESQP 300
    SMPAFMPEFQ GGSYNPWGGP EGGCPGDIGD DFANLFYRWN IGQRVTAMSL 350
    YMMFGGQNPG AMAAPVTASS YDYSAPISED RSIWSKYHET KLLALFTRSA 400
    KDLTMTELMG NGTQYTDNPA VRAYELRNPE TNSAFYATFH SNTSISTNEP 450
    FHLKVNTSAG VLTVPKYAST IRLNGHQSKI IVTDFTFGSK SLLYSTAEVL 500
    TYAVFDKKPT LVLWVPTGES GEFSIKGAKK GSIKKCQGCS RVKFIKEHGG 550
    LTTSLTQSAG MTVLEFDDGV RVILLDRTSA YDFWAPALTN DPFVPETESV 600
    LIQGPYLVRD AKLSGSKLAI TGDVVNATTL DVFAPKGVKS VTWNGKKVDT 650
    HSTEYGSLKG SLDAPQSIKL PALASWKSKD SLPERFADYD DSGAAWVDAN 700
    HMTTLNPRTP TSLPVLYADQ YGFHNGVRLW RGYFNGTATG AFINVQGGSA 750
    FGWSAWLNGE FLASHLGNAT TSQANLSLSF TDATLHTDTP NVLLIVHDDT 800
    GHDQTTGALN PRGIMDAKLL GSDSGFTHWR LAGTAGGESD LDPVRGVYNE 850
    DGLFAERVGW HLPGFDDSDW GEEGSAKDST TSVLSFEGAT VRFFRTTCPL 900
    DIPAHTDVSI SFVLSTPAGA TTEYRAQLFV NGYQYGRYNP YIGNQVVYPV 950
    PVGILDYKGE NTIGVAVWAQ SEEGASIGID WRVNYLADSS LDVASWDTKD 1000
    LRPGWTEERV KYA 1013
    Length:1,013
    Mass (Da):111,429
    Last modified:July 13, 2010 - v2
    Checksum:iA4858AD5E5C13BB2
    GO

    Sequence cautioni

    The sequence CAP79053.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM920421 Genomic DNA. Translation: CAP79053.1. Sequence problems.
    RefSeqiXP_002556674.1. XM_002556628.1.

    Genome annotation databases

    GeneIDi8315677.
    KEGGipcs:Pc06g00600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM920421 Genomic DNA. Translation: CAP79053.1 . Sequence problems.
    RefSeqi XP_002556674.1. XM_002556628.1.

    3D structure databases

    ProteinModelPortali B6GW04.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 500485.B6GW04.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8315677.
    KEGGi pcs:Pc06g00600.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OrthoDBi EOG7ZGXBD.

    Enzyme and pathway databases

    BioCyci PCHR:PC06G00600-MONOMER.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 28089 / DSM 1075 / Wisconsin 54-1255.

    Entry informationi

    Entry nameiBGALB_PENCW
    AccessioniPrimary (citable) accession number: B6GW04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 43 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3