B6EXY6 (LUP4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-amyrin synthase
Short name=AtBAS
EC=5.4.99.39

Alternative name(s):
Lupeol synthase 4
Short name=AtLUP4

Gene names

Name:	BAS
Synonyms:	LUP4
Ordered Locus Names:	At1g78950
ORF Names:	YUP8H12R.44

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts oxidosqualene to beta-amyrin. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin. Ref.4
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 3 PFTB repeats.
Sequence caution	The sequence AAC17080.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g78950 has been split into 2 genes: At1g78950 and At1g78955.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	beta-amyrin synthase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Beta-amyrin synthase		PRO_0000366134
Regions
	Repeat	149 – 190	42	PFTB 1
	Repeat	592 – 632	41	PFTB 2
	Repeat	641 – 682	42	PFTB 3
Experimental info
	Sequence conflict	334	1	P → L in BAG82628. Ref.1
	Sequence conflict	544	1	L → S in BAG82628. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B6EXY6 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: E8677A08B251C33D	FASTA	759	86,953
	10 20 30 40 50 60 MWRLKIGEGN GDDPYLFTTN NFAGRQTWEF DPDGGSPEER HSVVEARRIF YDNRFHVKAS 70 80 90 100 110 120 SDLLWRMQFL REKKFEQRIA PVKVEDSEKV TFETATSALR RGIHFFSALQ ASDGHWPAEN 130 140 150 160 170 180 AGPLFFLPPL VFCLYITGHL DEVFTSEHRK EILRYIYCHQ KEDGGWGLHI EGHSTMFCTT 190 200 210 220 230 240 LNYICMRILG ESPDGGHDNA CGRAREWILS HGGVTYIPSW GKTWLSILGV FDWSGSNPMP 250 260 270 280 290 300 PEFWILPSFF PVHPAKMWSY CRMVYLPMSY LYGKRFVGPI TSLILQLRKE LYLQPYEEIN 310 320 330 340 350 360 WMKVRHLCAK EDTYYPRPLV QELVWDSLYI FAEPFLARWP FNKLLREKAL QLAMKHIHYE 370 380 390 400 410 420 DENSRYITIG CVEKVLCMLA CWVEDPNGDY FKKHLSRISD YLWMAEDGMK MQSFGSQLWD 430 440 450 460 470 480 TGFAMQALLA SNLSSEISDV LRRGHEFIKN SQVGENPSGD YKSMYRHISK GAWTFSDRDH 490 500 510 520 530 540 GWQVSDCTAH GLKCCLLFSM LAPDIVGPKQ DPERLHDSVN ILLSLQSKNG GMTAWEPAGA 550 560 570 580 590 600 PKWLELLNPT EMFSDIVIEH EYSECTSSAI QALSLFKQLY PDHRTTEITA FIKKAAEYLE 610 620 630 640 650 660 NMQTRDGSWY GNWGICFTYG TWFALAGLAA AGKTFNDCEA IRKGVQFLLA AQKDNGGWGE 670 680 690 700 710 720 SYLSCSKKIY IAQVGEISNV VQTAWALMGL IHSGQAERDP IPLHRAAKLI INSQLESGDF 730 740 750 PQQQATGVFL KNCTLHYAAY RNIHPLWALA EYRARVSLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a product specific beta-amyrin synthase from Arabidopsis thaliana." Shibuya M., Katsube Y., Otsuka M., Zhang H., Tansakul P., Xiang T., Ebizuka Y. Plant Physiol. Biochem. 47:26-30(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana." Husselstein-Muller T., Schaller H., Benveniste P. Plant Mol. Biol. 45:75-92(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, CATALYTIC ACTIVITY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB374428 mRNA. Translation: BAG82628.1. AC002986 Genomic DNA. Translation: AAC17080.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE36183.1.
IPI	IPI00545484.
PIR	T01060.
RefSeq	NP_178016.2. NM_106544.4.
UniGene	At.34208.
3D structure databases
ProteinModelPortal	B6EXY6.
SMR	B6EXY6. Positions 99-280.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G78950.1-P.
Proteomic databases
PaxDb	B6EXY6.
PRIDE	B6EXY6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G78950.1; AT1G78950.1; AT1G78950.
GeneID	844234.
KEGG	ath:AT1G78950.
Organism-specific databases
TAIR	At1g78950.
Phylogenomic databases
eggNOG	COG1657.
HOGENOM	HOG000234317.
InParanoid	Q3ECA8.
KO	K15813.
OMA	FFADIVI.
PhylomeDB	B6EXY6.
ProtClustDB	PLN03012.
Gene expression databases
Genevestigator	B6EXY6.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUP4_ARATH

Accession

Primary (citable) accession number: B6EXY6
Secondary accession number(s): O64553, Q3ECA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program