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B6EXY6 (LUP4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-amyrin synthase

Short name=AtBAS
EC=5.4.99.39
Alternative name(s):
Lupeol synthase 4
Short name=AtLUP4
Gene names
Name:BAS
Synonyms:LUP4
Ordered Locus Names:At1g78950
ORF Names:YUP8H12R.44
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts oxidosqualene to beta-amyrin. Ref.1

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin. Ref.4

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 3 PFTB repeats.

Sequence caution

The sequence AAC17080.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g78950 has been split into 2 genes: At1g78950 and At1g78955.

Ontologies

Keywords
   DomainRepeat
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionbeta-amyrin synthase activity

Inferred from direct assay Ref.1. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759Beta-amyrin synthase
PRO_0000366134

Regions

Repeat149 – 19042PFTB 1
Repeat592 – 63241PFTB 2
Repeat641 – 68242PFTB 3

Experimental info

Sequence conflict3341P → L in BAG82628. Ref.1
Sequence conflict5441L → S in BAG82628. Ref.1

Sequences

Sequence LengthMass (Da)Tools
B6EXY6 [UniParc].

Last modified March 3, 2009. Version 2.
Checksum: E8677A08B251C33D

FASTA75986,953
        10         20         30         40         50         60 
MWRLKIGEGN GDDPYLFTTN NFAGRQTWEF DPDGGSPEER HSVVEARRIF YDNRFHVKAS 

        70         80         90        100        110        120 
SDLLWRMQFL REKKFEQRIA PVKVEDSEKV TFETATSALR RGIHFFSALQ ASDGHWPAEN 

       130        140        150        160        170        180 
AGPLFFLPPL VFCLYITGHL DEVFTSEHRK EILRYIYCHQ KEDGGWGLHI EGHSTMFCTT 

       190        200        210        220        230        240 
LNYICMRILG ESPDGGHDNA CGRAREWILS HGGVTYIPSW GKTWLSILGV FDWSGSNPMP 

       250        260        270        280        290        300 
PEFWILPSFF PVHPAKMWSY CRMVYLPMSY LYGKRFVGPI TSLILQLRKE LYLQPYEEIN 

       310        320        330        340        350        360 
WMKVRHLCAK EDTYYPRPLV QELVWDSLYI FAEPFLARWP FNKLLREKAL QLAMKHIHYE 

       370        380        390        400        410        420 
DENSRYITIG CVEKVLCMLA CWVEDPNGDY FKKHLSRISD YLWMAEDGMK MQSFGSQLWD 

       430        440        450        460        470        480 
TGFAMQALLA SNLSSEISDV LRRGHEFIKN SQVGENPSGD YKSMYRHISK GAWTFSDRDH 

       490        500        510        520        530        540 
GWQVSDCTAH GLKCCLLFSM LAPDIVGPKQ DPERLHDSVN ILLSLQSKNG GMTAWEPAGA 

       550        560        570        580        590        600 
PKWLELLNPT EMFSDIVIEH EYSECTSSAI QALSLFKQLY PDHRTTEITA FIKKAAEYLE 

       610        620        630        640        650        660 
NMQTRDGSWY GNWGICFTYG TWFALAGLAA AGKTFNDCEA IRKGVQFLLA AQKDNGGWGE 

       670        680        690        700        710        720 
SYLSCSKKIY IAQVGEISNV VQTAWALMGL IHSGQAERDP IPLHRAAKLI INSQLESGDF 

       730        740        750 
PQQQATGVFL KNCTLHYAAY RNIHPLWALA EYRARVSLP 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a product specific beta-amyrin synthase from Arabidopsis thaliana."
Shibuya M., Katsube Y., Otsuka M., Zhang H., Tansakul P., Xiang T., Ebizuka Y.
Plant Physiol. Biochem. 47:26-30(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana."
Husselstein-Muller T., Schaller H., Benveniste P.
Plant Mol. Biol. 45:75-92(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, CATALYTIC ACTIVITY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB374428 mRNA. Translation: BAG82628.1.
AC002986 Genomic DNA. Translation: AAC17080.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36183.1.
PIRT01060.
RefSeqNP_178016.2. NM_106544.4.
UniGeneAt.34208.

3D structure databases

ProteinModelPortalB6EXY6.
SMRB6EXY6. Positions 11-757.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G78950.1-P.

Proteomic databases

PaxDbB6EXY6.
PRIDEB6EXY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G78950.1; AT1G78950.1; AT1G78950.
GeneID844234.
KEGGath:AT1G78950.

Organism-specific databases

TAIRAT1G78950.

Phylogenomic databases

eggNOGCOG1657.
HOGENOMHOG000234317.
InParanoidQ3ECA8.
KOK15813.
OMAFFADIVI.
PhylomeDBB6EXY6.
ProtClustDBPLN03012.

Gene expression databases

GenevestigatorB6EXY6.

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLUP4_ARATH
AccessionPrimary (citable) accession number: B6EXY6
Secondary accession number(s): O64553, Q3ECA8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names