B6ESC4 (BIOD_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	VSAL_II0857

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000119852
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
	Nucleotide binding	116 – 119	4	ATP By similarity
	Nucleotide binding	176 – 177	2	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	55	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ESC4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 2466FF262A2A868B	FASTA	227	24,387
	10 20 30 40 50 60 MIDAFFIAGT DTDVGKTVAS KAILDALNMK GLRTAAYKPI AAGSEDKGDG VQNSDAIHLR 70 80 90 100 110 120 SVANVELSYD EVNPYALLLP TSPHIAAAAE NIVIDYSVLS KGLASLKAKS DIVLVEGAGG 130 140 150 160 170 180 WRVPVSKSDC LSTWVQQEKL PVVLVVGIKL GCLSHAMLTE EAIKHDGLDI VGWVANRVNP 190 200 210 220 GTEHYADIIE MLEDKMPAPK LGEIPYMPSI KRKSMGKYIN LDSLMEI

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178380 Genomic DNA. Translation: CAQ81611.1.
RefSeq	YP_002265159.1. NC_011313.1.
3D structure databases
ProteinModelPortal	B6ESC4.
ModBase	Search...
Protein-protein interaction databases
STRING	B6ESC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6962202.
GenomeReviews	Gene locus VSAL_II0857 in contig FM178380_GR.
KEGG	vsa:VSAL_II0857.
PATRIC	20858534. VBIAliSal95923_4218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	TRTNEDV.
ProtClustDB	PRK00090.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_ALISL

Accession

Primary (citable) accession number: B6ESC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program