B6ERM8 (NADE_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	VSAL_II0607

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000098999
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
Sites
	Active site	45	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ERM8 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: EFA5DBD5442224E3	FASTA	276	30,256
	10 20 30 40 50 60 MQKQIVEEMK VKVSIDPLSE IQRRVDFIKS TLTQSGCKSL ILGISGGVDS TTCGRLAQIA 70 80 90 100 110 120 VNELNKESNS SDYQFIAVRL PYGIQKDEDE AQLALEFIKP THSISINIKD GVDGLHKANH 130 140 150 160 170 180 LGLAHTGLLP TANDKIDFVK GNVKARARMI AQYEVAGYVG GLVLGTDHSA ENITGFYTKF 190 200 210 220 230 240 GDGACDLAPL FGLNKRQVRD VAAELGAPEQ LVKKVPTADL EELAPQKADE DALSVTYDEI 250 260 270 DDFLEGKKID TEAEARLIKI YQTSQHKRKP IPTIYD

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178380 Genomic DNA. Translation: CAQ81361.1.
RefSeq	YP_002264932.1. NC_011313.1.
3D structure databases
ProteinModelPortal	B6ERM8.
ModBase	Search...
Protein-protein interaction databases
STRING	B6ERM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6962001.
GenomeReviews	Gene locus VSAL_II0607 in contig FM178380_GR.
KEGG	vsa:VSAL_II0607.
PATRIC	20857993. VBIAliSal95923_3957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ALISL

Accession

Primary (citable) accession number: B6ERM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program