Reviewed, UniProtKB/Swiss-Prot B6ER91 (PYRC_ALISL)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	VSAL_II0468

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Dihydroorotase HAMAP MF_00219		PRO_1000100036
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	98	1	Zinc 1; via carbamate group By similarity
	Metal binding	98	1	Zinc 2; via carbamate group By similarity
	Metal binding	135	1	Zinc 2 By similarity
	Metal binding	173	1	Zinc 2 By similarity
	Metal binding	246	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	98	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ER91-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: DBA053A35DA66AC2	FASTA	342	37,790
	10 20 30 40 50 60 MTTLTITRPD DWHLHLRDGD VLTDTVRDSG RYNGRALIMP NLVPPVITTE QALSYRERIQ 70 80 90 100 110 120 AVNSSNTFAP IMSLYLTEKT TSDEIRKAKA TGYIVAAKLY PAGATTNSDS GVSDVQKVYP 130 140 150 160 170 180 ILKTMQEEGM LLLIHGEVTT HDIDIFDREK TFLDTVLAPI VNDFPELKIV LEHITTKDAA 190 200 210 220 230 240 DFVKNAGPNV AATITAHHLL FNRNHMLVGG IKPHFYCLPI LKRNTHQLAL IEAATSGSPK 250 260 270 280 290 300 FFLGTDSAPH SKEKKEAACG CAGSYTAHAS IELYTEVFEN EGKLDNLEAF ASFNGPDFYN 310 320 330 340 LPRNTDTITL VKEAWITPET MSFGNDVVVP IRAGEAVEWL VK

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178380 Genomic DNA. Translation: CAQ81222.1.
RefSeq	YP_002264799.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6961895.
GenomeReviews	Gene locus VSAL_II0468 in contig FM178380_GR.
KEGG	vsa:VSAL_II0468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IMPNLVP.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ALISL

Accession

Primary (citable) accession number: B6ER91

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)