B6EP33 (ILVC_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	VSAL_I0087

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000190902
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B6EP33 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 58EFE326D81E7294	FASTA	494	54,780
	10 20 30 40 50 60 MSNYFDTLNL REQLDQLGRC RFMDREEFST EADYLKGKRV VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LNVAYALRQA AIDEQRQSFK NASENGFEVA SYEKLIPQAD LVINLTPDKQ HTNVVETVMP 130 140 150 160 170 180 LMKEGATLGY SHGFNIVEEG MKIRKDLTVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPKGEGWDI AKAWAAGTGG HRAGCLESSF VAEVKSDLMG EQTILCGMLQ AGSIVCYEKM 250 260 270 280 290 300 IADGIDASYA GKLLQFGWET VTEALKFGGV THMMDRLSNP AKIKAFDLSE ELKDLMRPLY 310 320 330 340 350 360 NKHMDDIISG HFSSTMMADW ANDDVNLLGW REETGETAFE NYPDSSLEIP EQEYFDNGIL 370 380 390 400 410 420 MVAMVRAGVE LAFEAMTASG IVDESAYYES LHELPLIANT VARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 NYLFANVATP LLREKFMPLV DTDVIGRGLG AISNQVDNAK LIEVNETIRN HPVEYIGEEL 490 RGYMTDMKTI AVGG

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ77772.1.
RefSeq	YP_002261639.1. NC_011312.1.
3D structure databases
ProteinModelPortal	B6EP33.
ModBase	Search...
Protein-protein interaction databases
STRING	B6EP33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6988713.
GenomeReviews	Gene locus VSAL_I0087 in contig FM178379_GR.
KEGG	vsa:VSAL_I0087.
PATRIC	20850182. VBIAliSal95923_0170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ALISL

Accession

Primary (citable) accession number: B6EP33

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program