Reviewed, UniProtKB/Swiss-Prot B6ENU1 (PLSB_ALISL)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	VSAL_I2894

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123073
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B6ENU1-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 91EFE90897A1151C	FASTA	807	91,258
	10 20 30 40 50 60 MSTGQTIYHS LLKLPLSVMV KSTPIPSNPI EDLNIDIERP IIYALPFRSH VDLLTLQKSA 70 80 90 100 110 120 KELGLPDPLS PIEIDGVSYP RYVFTSIGPK MFDTDDDLPQ ESLDLFKIVL KHHADNPDAD 130 140 150 160 170 180 FQLIPTSILW GRRPGKEGTS RPHLMPLNGP QKFVTLIKAG RDSTVRISPV VSLRYMADNH 190 200 210 220 230 240 GADDAIAHKL ARVAKIHFSR QKLAASGPNL PNRQALFNRL LKSQAIEKVI LEEARIRNVD 250 260 270 280 290 300 VEKVRKEAMG IMEEIATNFS YSLIKNGNRI LKWLWNRLYQ GLNINNAATV RKLAQEGHEI 310 320 330 340 350 360 VYVPCHRSHM DYLLLSYVLY HEGLVPPHIA AGINLNFFPA GPIFRRGGAF FIRRSFKGNR 370 380 390 400 410 420 LYSTIFREYL AELFAKGYSV EYFSEGGRSR TGRLLQAKTG MLAMTVQAML RGLNRPVTLV 430 440 450 460 470 480 PVYIGYEHVM EVTTYAKELQ GKRKEKENAG QVLRTLRKLR NFGQGYVNFG EPISLNHYLN 490 500 510 520 530 540 EHAPNWSESI NPIEPQKPEW MSPVVNGIAN KMMTHINDAV AANALTLCAT ALLAARQRAL 550 560 570 580 590 600 SKEDLTEQLD CYLQLLRNIP YSNTATVPTQ DAEALLEHAI ALDKFVIEKD TLGEIISLDR 610 620 630 640 650 660 NQSILMTYYR NNIIHLFALP SLIAKLVVQY RSISIDNVQA QIQQIYPFLK AELFLHYDES 670 680 690 700 710 720 ELNDVVSQHI DELVRQKLIE RENDVLQLNA TNILKVHLLA HTISETLQRY AIALTHLQAS 730 740 750 760 770 780 PKLGKNDLEE QSQIMAQRLS RLHGINAPEF FDKGVFGILF NTLKAEGYLN SDGVAVISKV 790 800 EPFSRDMSRL LNPEIKLTIQ AVMTKED

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ80578.1.
RefSeq	YP_002264224.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988749.
GenomeReviews	Gene locus VSAL_I2894 in contig FM178379_GR.
KEGG	vsa:VSAL_I2894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_ALISL

Accession

Primary (citable) accession number: B6ENU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)