Reviewed, UniProtKB/Swiss-Prot B6ENN5 (SYS_ALISL)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	VSAL_I1844

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Seryl-tRNA synthetase HAMAP MF_00176		PRO_1000098029
Regions
	Nucleotide binding	273 – 275	3	ATP By similarity
	Nucleotide binding	360 – 363	4	ATP By similarity
	Region	242 – 244	3	Serine binding By similarity
Sites
	Binding site	296	1	Serine By similarity
	Binding site	396	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ENN5-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 06947C16EBD98587	FASTA	435	49,009
	10 20 30 40 50 60 MLDSKLLRTE LDETAEKLAR RGFKLDVETL RTLEEQRKSL QVITEDLQAQ RNSRSKSIGQ 70 80 90 100 110 120 AKAKGDHEEA DRIMADVATL GSELDDAKAG LAELQQQIES IALSVPNLPD ESVPYGKDED 130 140 150 160 170 180 ENVEVLRWGT PLTYDFEVRD HVALGELADG LDFASAVKIS GSRFIVMKGQ FARLHRALSQ 190 200 210 220 230 240 FMLDLHTEEH GYMEMYVPYL VNHDSLYGTG QLPKFGEDLF HTSPLTEQVS DVPLKMLSLI 250 260 270 280 290 300 PTAEVPVTNM VRDTITEEAD LPLKMTAHTP CFRSEAGSYG RDTRGLIRMH QFDKVELVQI 310 320 330 340 350 360 TKPEDSMAAL EELTGHAEKV LQLLELPYRK VILCTGDMGF GSRKTYDLEV WVPAQETYRE 370 380 390 400 410 420 ISSCSNMWDF QARRMQARFR RQGEKKPELV HTLNGSGLAV GRTMVAILEN FQQADGKIAI 430 PEVLRKYMNG VEFIG

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ79529.1.
RefSeq	YP_002263240.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6987417.
GenomeReviews	Gene locus VSAL_I1844 in contig FM178379_GR.
KEGG	vsa:VSAL_I1844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LKPYMGG.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_ALISL

Accession

Primary (citable) accession number: B6ENN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)