Reviewed, UniProtKB/Swiss-Prot B6ENA8 (PUR9_ALISL)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	VSAL_I2842

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000096037

Sequences

	Sequence		Length	Mass (Da)
	B6ENA8-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 629A91612FCC0E68	FASTA	530	57,624
	10 20 30 40 50 60 MNNPRPIRRA LISVSDKTGI VEFAQALTER GVDILSTGGT ARLLAEKNIP VTEVSDYTGF 70 80 90 100 110 120 PEMMDGRVKT LHPKVHGGVL GRRGQDDGIM EQHGINPIDI VVVNLYPFAE TVAKEGCTLA 130 140 150 160 170 180 DAVENIDIGG PTMVRSAAKN HKDVTIVVNA HDYDRVIAEM DTNDKSLTQA TRFDLAIAAF 190 200 210 220 230 240 EHTASYDGMI ANYFGTMVPS YGENKEGDNE SKFPRTFNQQ FEKKQDMRYG ENSHQAAAFY 250 260 270 280 290 300 VEANPEEASV STARQIQGKA LSYNNIADTD AALECVKEFN EPACVIVKHA NPCGVALGKD 310 320 330 340 350 360 VLEAYHRAYQ TDPTSAFGGI IAFNRELDAA TATAITERQF VEVIIAPSVS AEAIEIIAAK 370 380 390 400 410 420 KNLRLLECGE WSTKTTEFDI KRVNGGLLVQ DRDQGMVSED ELEVVSKRQP TAEELKDALF 430 440 450 460 470 480 CWKVAKYVKS NAIVYSKGDM TIGVGAGQMS RVYSAKIAGI KAADEGLVVE GCAMASDAFF 490 500 510 520 530 PFRDGIDAAA EAGIKCVIQP GGSMRDDEVI AAADEHGMAM IFTGMRHFRH

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ80526.1.
RefSeq	YP_002264176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6987941.
GenomeReviews	Gene locus VSAL_I2842 in contig FM178379_GR.
KEGG	vsa:VSAL_I2842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_ALISL

Accession

Primary (citable) accession number: B6ENA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	February 9, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)