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B6EMY6

- SYI_ALISL

UniProt

B6EMY6 - SYI_ALISL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei576 – 5761Aminoacyl-adenylateUniRule annotation
    Binding sitei620 – 6201ATPUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi935 – 9351ZincUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:VSAL_I0580
    OrganismiAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238))
    Taxonomic identifieri316275 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    ProteomesiUP000001730: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000189119Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi316275.VSAL_I0580.

    Structurei

    3D structure databases

    ProteinModelPortaliB6EMY6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi617 – 6215"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B6EMY6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKETLNL PETGFPMRGN LANREPVMLK HWYDEDLYGE IRKAKKGKKS    50
    FILHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YIPGWDCHGL 100
    PIELMVEKKK GKPGQKISAA EFREECRKYA AVQVEGQKES FKRLGIMGEW 150
    DKPYRTMDFG TEANIIRSLG KIADQGHLLK GFKPVHWCTD CGSALAEAEV 200
    EYQDKFSPSI DVRFVAADEA ATLAKFSTPE GHQGEGELSV VIWTTTPWTL 250
    PANRAVALHA DLEYVLVQVE AHGEQKAERL ILASELAKSV MDRAGIEHFH 300
    NLGFAKGSEL ELLQFNHPFY GFTVPAILGE HVTVDSGTGI VHTAPGHGQE 350
    DFVVGKKYDL EIANPVGSNG VYLPDTELFA GQHVFKANDS VIDVLKEKGA 400
    LLHHHAIEHS YPHCWRHKTP IIFRATPQWF ISMDQAGLRA KALEEVKSVE 450
    WMPEWGQSRI EGMIEGRPEW CISRQRTWGV PIALFVHKET SELHPDSLEL 500
    IEKVAKLVEE KGIQAWWDLD IAELMGEDDA TKYEKVLDTL DVWFDSGVTH 550
    FSVVDSREEY NFPQEERTHS ADLYLEGSDQ HRGWFQSSLI SSVAMKGKAP 600
    YRQVLTHGFV VDGNGRKMSK SVGNVVAPKD VTNKLGADIL RLWVASTDYT 650
    NEVAVSDEIL KRSADAYRRI RNTARFFLAN LNGFNPETDI VPAEEMVALD 700
    RWAVGRAFAA QEEIIKSYDE YNLHEVTQRL MHFCSIEMGS FYLDVIKDRQ 750
    YTAKKSGHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW NEMPGKRDKF 800
    VFTGEWYEGL FDLAEGEDLN NEFWSKIQAV RASVNKLLEA ARGEKVIGGS 850
    LQAEITLYAD DALAAKINKL DNELRFVLLT SSATVKPLSE KSDSAKATEL 900
    DGLFVDVAAS EAAKCERCWH HVADVGTIEG HEEVCGRCVS NVEGEGEERK 950
    FA 952
    Length:952
    Mass (Da):106,898
    Last modified:November 25, 2008 - v1
    Checksum:iA0926B7211DC6395
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM178379 Genomic DNA. Translation: CAQ78265.1.
    RefSeqiYP_002262100.1. NC_011312.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ78265; CAQ78265; VSAL_I0580.
    GeneIDi6989100.
    KEGGivsa:VSAL_I0580.
    PATRICi20851263. VBIAliSal95923_0687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM178379 Genomic DNA. Translation: CAQ78265.1 .
    RefSeqi YP_002262100.1. NC_011312.1.

    3D structure databases

    ProteinModelPortali B6EMY6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316275.VSAL_I0580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ78265 ; CAQ78265 ; VSAL_I0580 .
    GeneIDi 6989100.
    KEGGi vsa:VSAL_I0580.
    PATRICi 20851263. VBIAliSal95923_0687.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238 shows extensive evidence of gene decay."
      Hjerde E., Lorentzen M.S., Holden M.T., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.P., Thomson N.R.
      BMC Genomics 9:616-616(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LFI1238.

    Entry informationi

    Entry nameiSYI_ALISL
    AccessioniPrimary (citable) accession number: B6EMY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3