B6EMT2 (AMPA_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	VSAL_I0526

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098303
Sites
	Active site	281	1	Potential
	Active site	355	1	Potential
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 1 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	292	1	Manganese 2 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EMT2 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 0C7FAB9734B7BEC1	FASTA	502	54,605
	10 20 30 40 50 60 MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISSLLRRG DLEGKPGQML 70 80 90 100 110 120 LLHQVPNILS ERVLLVGCGK ERELGERQYK DIIKKTISTL NETGSMEAVC FLTELHVKGR 130 140 150 160 170 180 DTYWKVRQAV ESTKDSLYTF NQFKSNKPET RRPLRKLVFN VPTRRELSLG EKAINHGLSI 190 200 210 220 230 240 SSGVKASKDL GNMPPNVANP AYLASQARRL ADDFETVTTK VIGEEEMKKL GMTSYLAVGQ 250 260 270 280 290 300 GSHNESMMSV MEYKGHPNPD AKPIVLIGKG LTFDSGGISL KPSDSMDEMK YDMCGAASVF 310 320 330 340 350 360 GAMKALAKLN LPLNVVGILA GCENMPSSNS YRPGDILTTM SGQTVEVLNT DAEGRLVLCD 370 380 390 400 410 420 ALTYVERYEP DCVVDVATLT GACVVALGHH INGLLSNHNP LAHELINASE QSGDRAWRLP 430 440 450 460 470 480 MSEEYNEQLN SPFADMANIG GKAGGTITAG CFLARFAKKY NWAHIDSAGT AWVSGANKGS 490 500 TGRPVSLLVQ FLLNRSGPES VE

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ78211.1.
RefSeq	YP_002262047.1. NC_011312.1.
3D structure databases
ProteinModelPortal	B6EMT2.
ModBase	Search...
Protein-protein interaction databases
STRING	B6EMT2.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6988676.
GenomeReviews	Gene locus VSAL_I0526 in contig FM178379_GR.
KEGG	vsa:VSAL_I0526.
PATRIC	20851141. VBIAliSal95923_0634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	MPANICT.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ALISL

Accession

Primary (citable) accession number: B6EMT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program