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Protein

Argininosuccinate synthase

Gene

argG

Organism
Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argHA), Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei92CitrullineUniRule annotation1
Binding sitei97CitrullineUniRule annotation1
Binding sitei122ATP; via amide nitrogenUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei128AspartateUniRule annotation1
Binding sitei128CitrullineUniRule annotation1
Binding sitei129AspartateUniRule annotation1
Binding sitei132CitrullineUniRule annotation1
Binding sitei181CitrullineUniRule annotation1
Binding sitei190CitrullineUniRule annotation1
Binding sitei266CitrullineUniRule annotation1
Binding sitei278CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 21ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:VSAL_I2758
OrganismiAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238))
Taxonomic identifieri316275 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000001730 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000890211 – 403Argininosuccinate synthaseAdd BLAST403

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi316275.VSAL_I2758.

Structurei

3D structure databases

ProteinModelPortaliB6EMN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6EMN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKVNVNKV VVAYSGGLDT SVIIPWLKEN YDCEVIAFVA DVGQGAEELE
60 70 80 90 100
GIEAKAIASG ASECYVADLK EEMVSEYIFP TLKTGALYEG KYLLGTSMAR
110 120 130 140 150
PIIAKAQVEV ARNVGADALC HGCTGKGNDQ IRFEGAFAAL APDLHVIAPW
160 170 180 190 200
REWDLVSREE CLDYLAERNI PCTASLTKIY SRDANAWHIS TEGGVLEDTW
210 220 230 240 250
NEPNEDCWAW TVDPEQAPNE SETVSIKVEK GAVVAVDGKA MTPYEVVVYL
260 270 280 290 300
NEKGIKHGVG RIDIVENRLV GMKSRGCYET PGGTIINEAL RAVEQLVLDK
310 320 330 340 350
ASFEFREELG IKASHLVYDG RWFTPLCKSI LAATEELAQD VNGDVVIKLY
360 370 380 390 400
KGQATVTQKR SDNSLYSEEF ATFGADEVYD QSHAEGFIRL YSLSSRIRAL

NSK
Length:403
Mass (Da):44,340
Last modified:November 25, 2008 - v1
Checksum:i4BFC2888DC934D2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM178379 Genomic DNA. Translation: CAQ80442.1.
RefSeqiWP_012551198.1. NC_011312.1.

Genome annotation databases

EnsemblBacteriaiCAQ80442; CAQ80442; VSAL_I2758.
KEGGivsa:VSAL_I2758.
PATRICi20856006. VBIAliSal95923_2991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM178379 Genomic DNA. Translation: CAQ80442.1.
RefSeqiWP_012551198.1. NC_011312.1.

3D structure databases

ProteinModelPortaliB6EMN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316275.VSAL_I2758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ80442; CAQ80442; VSAL_I2758.
KEGGivsa:VSAL_I2758.
PATRICi20856006. VBIAliSal95923_2991.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_ALISL
AccessioniPrimary (citable) accession number: B6EMN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: October 5, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.