Reviewed, UniProtKB/Swiss-Prot B6ELH8 (MRAY_ALISL)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	VSAL_I2647

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000090586
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	168 – 188	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	236 – 256	21	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	288 – 308	21	Potential
	Transmembrane	338 – 358	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B6ELH8-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 399C221BBBC530CA	FASTA	360	40,208
	10 20 30 40 50 60 MIIWLSNFLE QYFPFFRLFE YLSFRAILSI ITALSISLWM GPKLIRWLQN LQIGQVVRDD 70 80 90 100 110 120 GPESHFSKRG TPTMGGIMIL TAITVTVFLW ADLTNPYVWA VMFVLLGYGA VGFVDDYRKV 130 140 150 160 170 180 VRKNTDGLIA RWKYFWQSAI ALVVAFALYA YGKDTAATQL VVPFFKDIMP QLGLTYILLT 190 200 210 220 230 240 YFVIVGTSNA VNLTDGLDGL AIMPTVFVAA GFAFIAWATG NVQFANYLHI PHIPQASELV 250 260 270 280 290 300 VVCAAIVGAG FGFLWFNTYP AQVFMGDVGS LALGGALGTI AVLVRQEFLL VIMGGVFVVE 310 320 330 340 350 360 TLSVILQVGS YKLRGQRIFR MAPIHHHYEL KGWPEPRVIV RFWIISMLLV LIALATLKVR

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ80331.1.
RefSeq	YP_002263985.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988861.
GenomeReviews	Gene locus VSAL_I2647 in contig FM178379_GR.
KEGG	vsa:VSAL_I2647.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ALISL

Accession

Primary (citable) accession number: B6ELH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)