Reviewed, UniProtKB/Swiss-Prot B6ELH4 (MURC_ALISL)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	VSAL_I2643

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000091075
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B6ELH4-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 55BFE944DCB68F86	FASTA	487	53,279
	10 20 30 40 50 60 MTTEQKLQLA EIRTMIPEMR RVECIHFVGI GGAGMSGIAE VLLNEGYHIS GSDVAENSVT 70 80 90 100 110 120 VRLAEKGADI FFGHHASNVE KASVVVVSTA IDQANPEIVA AKENRIPVIR RAEMLAELMR 130 140 150 160 170 180 YRHGIAVAGT HGKTTTTALT TQIYSEAGLD PTFVNGGLVK NAGTNARLGS SRFLIAEADE 190 200 210 220 230 240 SDASFLHLQP MVSIVTNIEA DHMDTYGGDF EVLKQTFIDF LHNLPFYGQA VMCIDDDVVR 250 260 270 280 290 300 ELLPRVSRQV ITYGFSDDAD VRLINYRQEG QKSFFTVQRK DRTDLDIVLN IPGKHNALNA 310 320 330 340 350 360 TAAIAVATEE DVEDSAILSA LLNFEGAGRR FDQLGEFETG NGHAMLVDDY GHHPTEVDVT 370 380 390 400 410 420 IKAARAGWED KRLVMIFQPH RYSRTRDLYD DFANVLDNVD VLIMLDVYSA GEKPIAGADG 430 440 450 460 470 480 RALCRTIRAR GKLDPIFVPT IDALPSVLAN IIQNNDLVLT QGAGDVGKLA KQLASLELNI QAMKELG

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ80327.1.
RefSeq	YP_002263981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988571.
GenomeReviews	Gene locus VSAL_I2643 in contig FM178379_GR.
KEGG	vsa:VSAL_I2643.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ALISL

Accession

Primary (citable) accession number: B6ELH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)